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P4682

Phénol solution

Name: Phénol solution
Brand: SIGMA

Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology

Synonyme(s) :

nucleic acid extraction

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Conditionnement	Référence	Disponibilité	Prix

A propos de cet article

Formule empirique (notation de Hill) :

C₆H₆O

Numéro CAS:

108-95-2

Poids moléculaire :

94.11

UNSPSC Code:

12352104

NACRES:

NA.52

PubChem Substance ID:

57654523

MDL number:

MFCD00002143

Beilstein/REAXYS Number:

6428463

Grade:

Molecular Biology

Technique(s):

RNA purification: suitable

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Propriétés

grade

Molecular Biology

Quality Segment

200

product line

BioReagent

form

liquid

technique(s)

RNA purification: suitable

pH

4.1-4.5 (25 °C)

storage temp.

2-8°C

SMILES string

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Gene Information

human ... GABRA1(2554)

Description

General description

Phenol solution is a clear liquid saturated with citrate buffer. Phenols are organic compounds that feature a hydroxyl group directly bonded to an aromatic carbocyclic ring. The common name for monohydroxybenzene is phenol.

Application

Phenol solution has been used:

for the extraction of RNA by standard phenol-chloroform protocols
in cell wall monosaccharide analysis to treat epidermal strips from the abaxial side of Commelina communisin leaves[1]
as a component of lysis-denaturation buffer for RNA isolation

Biochem/physiol Actions

Phenol solution can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol-chloroform protocols. This organic solvent also serves as a potential protein denaturant. Phenol-chloroform extraction is a broadly used method for the extraction of nucleic acids. Despite phenol′s incomplete inhibition of RNase activity, it possesses characteristics that make it useful for isolating nucleic acids when combined with chloroform and alcohol.

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Cet article	P4557	48688	40063
Sigma-Aldrich P4682 Phenol solution	Sigma-Aldrich P4557 Phenol solution	Supelco 48688 Phenol solution	Supelco 40063 Phenol solution
technique(s) RNA purification: suitable	technique(s) -	technique(s) HPLC: suitable, gas chromatography (GC): suitable	technique(s) HPLC: suitable, gas chromatography (GC): suitable
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 300
grade Molecular Biology	grade Molecular Biology	grade certified reference material, TraceCERT^®	grade certified reference material, TraceCERT^®
form liquid	form liquid	form -	form -
pH 4.1-4.5 (25 °C)	pH 7.7-8.1 (25 °C)	pH -	pH -
product line BioReagent	product line BioReagent	product line TraceCERT^®	product line TraceCERT^®