Mixing the two phenol layers does not damage this product. Phase separation can occur during handling. If layers mix, allow the solution to stand until a clear phase separation is re-established before pipetting. Temporary haziness or a visible interface after mixing is normal. Pipetting should occur only after full separation to avoid aqueous carryover and ensure consistent performance. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/154/087/p4557dat.pdf
P4557
Phénol solution
BioReagent, Molecular Biology
Synonyme(s) :
Tris-equilabrated Phenol
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A propos de cet article
Formule linéaire :
C6H5OH
Numéro CAS:
Poids moléculaire :
94.11
UNSPSC Code:
12352104
NACRES:
NA.52
PubChem Substance ID:
EC Number:
203-632-7
Beilstein/REAXYS Number:
6428463
MDL number:
Assay:
≥99.0% (GC)
Grade:
Molecular Biology
Le tarif et la disponibilité ne sont pas disponibles actuellement.
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Molecular Biology
Quality Segment
product line
BioReagent
assay
≥99.0% (GC)
form
liquid
dilution
(for analytical testing)
pH
7.7-8.1 (25 °C)
storage temp.
2-8°C
SMILES string
Oc1ccccc1
InChI
1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
InChI key
ISWSIDIOOBJBQZ-UHFFFAOYSA-N
Gene Information
human ... GABRA1(2554)
General description
Phenol solution is a clear liquid and can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.
Application
Phenol solution has been used:
- as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
- for extraction of DNA from Arabidopsis thaliana Rosette leaves
- in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
- as a component of extraction buffer to extract RNA from fruit pulp
- for the extraction of protein from roots
- for the isolation of fecal DNA
- for the extraction of caged RNA[1]
Biochem/physiol Actions
The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.
Other Notes
- 1 bottle Tris-equilabrated Phenol (product number P4557)
- Equilibration Buffer (product B5658)
1 of 1
Cet article | |||
|---|---|---|---|
| assay ≥99.0% (GC) | assay - | assay - | assay - |
| grade Molecular Biology | grade Molecular Biology | grade certified reference material, TraceCERT® | grade certified reference material, TraceCERT® |
| form liquid | form liquid | form - | form - |
| pH 7.7-8.1 (25 °C) | pH 4.1-4.5 (25 °C) | pH - | pH - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-30°C | storage temp. 2-8°C |
| product line BioReagent | product line BioReagent | product line TraceCERT® | product line TraceCERT® |
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signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
Classe de stockage
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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What happens if I mix the two layers of phenol before pipetting? I prepared FIAC this way
1 answer-
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How long can you store phenol for, after adding the buffer?
1 answer-
Following the addition of buffer, the solution may be stored for 6 months at 2–8 °C. For extended storage after opening, with or without the addition of buffer, the material may be stored at –20 °C, protected from light. This information is available in the product datasheet. Please see the link below to review additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/154/087/p4557dat.pdfHelpful?
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Esta botella de Fenol (10 mM TrisHCl pH 8.0) viene junto con una botella pequeña de tampón Tris a pH 10.5 for saturated phenol. Se supone que debo añadírsela a la botella de fenol para ajustar a 10 mM TrisHCl?
1 answer-
Yes, this phenol solution includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. The entire contents of the bottle of the buffer should be added to the phenol.
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What is the export packaging? Is it in thermocol/Foam packaging? Can you share the packaging Image.
1 answer-
Both package sizes are bottles that are sealed in a polyamide bag. This bag is placed, with additional absorbent material and padding, in a metal can with a pull tap. The final packaged item is placed in the shipping box that is most compatible with the order. This product is shipped at ambient temperature.
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I see that there are two layers in the bottle Phenol solution, Product P4557. Which layer do I use?
1 answer-
The top phase is Tris buffer, so use the lower/bottom phase.
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What is the molarity of Phenol solution, Product P4557?
1 answer-
The Molarity is ~10.6. The density of the phenol is 1.05g/ml, and the molecular weight is 94.11.
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What is the final pH of Phenol solution, Product P4557, after adding the buffer?
1 answer-
The pH is typically 8.0; the specifications state a range of 7.7-8.1.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What do I do to equilibrate the Phenol solution, Product P4557, to around pH 8?
1 answer-
This phenol solution (Product No. P4557) includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. After addition of the buffer to the phenol, it may take about 6 hours (mixture can be kept at 2-8 °C) until the bottom phase (phenol) becomes clear. Some of the haziness in the phenol phase may be due to some air bubbles caused by mixing by inversion. There could be a hazy interface between the phenol and aqueous phase which remains after the separation. The entire contents of the bottle of the buffer should be added to the phenol. If buffering less than the complete bottle, use at a ratio of 6.5 mL buffer to 100 mL phenol. For 10 mL of phenol, use 650 μL of buffer. For 1 mL of phenol, use 65 μL of buffer.
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