The contents of the Tris base are added to the phenol:chloroform:isoamyl alcohol mixture, and the contents are well mixed. The amount added is 6.5 mL equilibration buffer per 100 mL phenol, designed so that only one treatment is necessary to raise the pH. Allow the mixture to separate, which may take 2-4 hours. The upper aqueous phase is removed and discarded. The remaining lower organic phase is divided into aliquots and stored at -20 °C. The solution should be stable for up to 12 months. The pH of the phenol should be raised from 6.7 to about 7.9-8. Only one treatment is needed.
P2069
Phénol:Chloroform:Alcool isoamylique 25:24:1 saturé avec 10 mM Tris, pH 8,0, 1 mM EDTA
Supplied with Equilibration buffer, Molecular Biology
Synonyme(s) :
DNA extraction
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A propos de cet article
NACRES:
NA.52
UNSPSC Code:
12190000
Assay:
≥99.0%
Grade:
Molecular Biology
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Molecular Biology
Quality Level
assay
≥99.0%
form
liquid
pH
6.5-6.9(pH of phenolic phase), 7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer)
application(s)
agriculture
storage temp.
2-8°C
Application
Ce lot de phénol:chloroforme:alcool isoamylique 25:24:1 a été testé par extraction d′une préparation brute d′ADN de pBR322 issu d′E. coli. Selon l′évaluation par électrophorèse sur gel d′agarose, l′ADN extrait n′était pas dégradé et sa concentration n′a pas été réduite. La même procédure a été appliquée à l′ADN purifié de pBR322 et à l′ADN de lambda incubé avec Hind III. Selon l′évaluation par électrophorèse, les préparations purifiées n′étaient pas dégradées de façon détectable et leur concentration n′a pas été réduite.
Le phénol:chloroforme:alcool isoamylique a été utilisé dans la préparation d′ARN pour étudier l′isolement d′ARN total à partir de biopsies du myocarde.[1] Il a également été utilisé pour extraire l′ADN dans les essais de clonalité du récepteur des androgènes humain (HUMARA).[2]
Pour les applications nécessitant un pH plus élevé, par exemple l′isolement d′ADN génomique intact de grande taille, l′ajout du tampon d′équilibration est recommandé.
Packaging
Ce produit est fourni avec P3803 et un flacon séparé de tampon d′équilibration pour ajuster la phase phénolique à pH 8,0 ± 0,2.
Preparation Note
Ajouter l′intégralité du petit flacon de tampon d′équilibration au grand flacon de phénol:chloroforme:alcool isoamylique. Mélanger doucement et laisser les phases se séparer avant utilisation, environ 2 à 4 heures. Ceci ajuste le pH de la solution de phénol de pH 6,7 ± 0,2 à 8,0 ± 0,2.
Other Notes
Après l′ajout de tampon d′équilibration, ce produit est stable pendant 6 mois (par rapport à 2 ans sans tampon ajouté). Un stockage à -20 °C à l′abri de la lumière prolonge la durée de conservation jusqu′à plus d′un an après ajout du tampon d′équilibration.
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Cet article | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| assay ≥99.0% | assay ≥99.0% | assay ≥99.0% | assay - |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| application(s) agriculture | application(s) agriculture | application(s) life science and biopharma | application(s) - |
| form liquid | form liquid | form liquid | form liquid |
| pH 6.5-6.9(pH of phenolic phase), 7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer) | pH 6.5-6.9(pH of phenolic phase) | pH 4.3-4.7 | pH 4.1-4.5 (25 °C) |
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signalword
Danger
Hazard Classifications
Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
target_organs
Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
Classe de stockage
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
Yongzhong Wang et al.
Microcirculation (New York, N.Y. : 1994), 13(3), 187-197 (2006-04-22)
Angiogenic therapies in animals have demonstrated the development of new blood vessels within ischemic myocardium. However, results from clinical protein and gene angiogenic trials have been less impressive. The present study aimed to investigate the expression of angiogenic genes in
Amber L Scott et al.
Molecular biology and evolution, 34(10), 2690-2703 (2017-09-29)
Polyploidization events have occurred during the evolution of many fungi, plant, and animal species and are thought to contribute to speciation and tumorigenesis, however little is known about how ploidy level contributes to adaptation at the molecular level. Here we
Martin Häring et al.
Frontiers in behavioral neuroscience, 9, 235-235 (2015-09-22)
The endocannabinoid (eCB) system possesses neuromodulatory functions by influencing the release of various neurotransmitters, including γ-aminobutyric acid (GABA) and glutamate. A functional interaction between eCBs and the serotonergic system has already been suggested. Previously, we showed that cannabinoid type-1 (CB1)
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| P2069-100ML | 04061837898709 |
| P2069-400ML | 04061837898723 |
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Hello, I used P2069 for DNA extraction from mouse tails. After adding the equilibration buffer, I wait for 2~4hrs until phase seperation. At this point, I have a question. I heard that lower phase is the phase I utilize, so Can I aliquot the lower phase?
1 answer-
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Hello, I used this material for DNA extraction from mouse tail. After mixing the equilibration buffer, there was two phase. Which phase should I use? Also, Can I aliquot the liquid?
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The lower phase should be used for DNA extraction. It is possible to aliquot the liquid immediately after mixing, before the phases separate.
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After equilibrating with the supplied Tris buffer, can I aliquot this for use, if so, should I just top each aliquot with additional Tris pH 8?
1 answer-
Please see the link below to review the product datasheet. Note that the prepration instructions require the addition of the entire vial (6.5 mL) of equilabration buffer to the contents of the product. Aliquot immediately after the gentle mixing step. During the 2 - 4 hour incubation period the mixure will phase separate. At this point the aliquots may be stored for long term use according to the instructions.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/144/892/p2069dat.pdfHelpful?
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There is an Equilibration Buffer (Product B5658) that is included with Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1. Is B5658 also available separately?
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No, it is only available as part of P2069.
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I see that you list two equilibrated Phenol:Chloroform:Isoamyl alcohol products. When should I use Product P3803, and when should I use Product P2069?
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In short, P3803 is for RNA work, and P2069 is for DNA work. Product P2069 consists of one bottle of Product P3803 and one bottle of Product B5658. The B5658 is added to the P3803 to raise the pH of the organic phase from 6.7 to 8.0; pH 8.0 is not suitable for RNA work, because RNA can degrade at the higher pH.
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I ordered Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1; I got one bottle of the Phenol:Chloroform:Isoamyl Alcohol and one bottle of B5658, Equilibration buffer. When and how am I supposed to use the B5658?
1 answer-
B5658 is a Tris buffer that is used to raise the pH of the phenol:chloroform:isoamyl alcohol mixture, to make it suitable for isolation of genomic DNA. The contents of the two bottles need to be well mixed. Then allow the mixture to separate; this may take 2-4 hours. This procedure should raise the pH of the phenol phase from 6.7 to about 8.0. Only one "treatment" is needed. This solution should be stable for up to 12 months.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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There are two layers in the bottle of Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1; which layer should I use?
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The lower phase is the phase that is to be used.
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