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P1944

Sigma-Aldrich

Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

for molecular biology

Synonyme(s) :

phenol chloroform RNA extraction

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About This Item

Code UNSPSC :
12190000
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Niveau de qualité

200

Pureté

≥99.0%

Forme

liquid

pH

4.3-4.7

Application(s)

life science and biopharma

Température de stockage

2-8°C

Description générale

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.

Application

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
  • for the extraction and purification of RNA.
  • to isolate RNA from Arabidopsis siliques.
  • to extract proteins and DNA from polysomal and non-polysomal fractions.
  • in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

Organes cibles

Liver,Kidney, Nervous system,Kidney,Liver,Skin

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

175.0 °F - closed cup

Point d'éclair (°C)

79.44 °C - closed cup

Équipement de protection individuelle

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Machine learning-assisted global DNA methylation fingerprint analysis for differentiating early-stage lung cancer from benign lung diseases
Lu D, et al.
Biosensors And Bioelectronics, 235, 115235-115235 (2023)
Exploring translation regulation by global analysis of ribosomal association
Melamed D, et al.
Methods in Enzymology, 48(3), 301-305 (2009)
Autophagy controls resource allocation and protein storage accumulation in Arabidopsis seeds
Berardino JD, et al.
Journal of Experimental Botany, 69(6), 1403-1414 (2018)
Molecular approaches for analysis of Drosophila MicroRNAs
Pandey M, et al.
Journal of Separation Science, 169-188 (2020)
An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
C Puissant et al.
BioTechniques, 8(2), 148-149 (1990-02-01)
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