This product is quite stable when stored in tablet form at 2-8°C. While storing the tablets at 20°C is not expected to be detrimental, stability studies have not been conducted. Upon reconstitution, prepared solutions are stable at least 2 weeks at 2-8°C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/262/291/s8830dat-ms.pdf
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A propos de cet article
Nom du produit
Cocktail d′inhibiteurs de protéases SIGMAFAST™, comprimés, sans EDTA, for use in purification of Histidine-tagged proteins
biological source
bovine lung
Quality Segment
form
tablet
storage temp.
2-8°C
General description
Application
Biochem/physiol Actions
Features and Benefits
Pepstatine A : Le cocktail contient une formule exclusive qui inclut un sel de pepstatine A, pour une protection supplémentaire qui n'est généralement pas possible en comprimé.
Vaste spécificité : Le cocktail inhibe les protéases à sérine, à cystéine et à acide aspartique ainsi que les métalloprotéases, assurant une large protection pour les échantillons de protéines.
Rendement élevé : Il est recommandé d'utiliser un comprimé, correspondant à 100 ml de cocktail, pour inhiber les protéases présentes dans une masse cellulaire maximale de 20 g, suspendue dans 100 ml d'extrait.
Stabilité de longue durée : Tels qu'ils sont fournis, les comprimés sont stables pendant au moins 2 ans entre 2 et 8 °C, et la solution reconstituée d'inhibiteurs de protéases (1x ou 10x) est stable pendant au moins 2 semaines entre 2 et 8 °C.
Physical form
Preparation Note
Other Notes
Bestatine
E-64
Pepstatine A
Phosphoramidon
Leupeptine
Aprotinine
Legal Information
1 of 1
Cet article | |||
|---|---|---|---|
| form tablet | form tablet | form DMSO solution | form tablet |
| biological source bovine lung | biological source bovine lung, bovine milk | biological source - | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
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Contenu apparenté
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| S8830-20TAB | 04061835518432 |
| S8830-2TAB | 04061835518449 |
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Can the tablets be stored at -20C? Any stability data around this?
1 answer-
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Can we use S8830-SIGMA FAST, EDTA-Free, tablet for plant extract also?
1 answer-
This product has not been tested for use with plants. For applications involving plants, product P9599, Protease Inhibitor Cocktail, is recommended for plant cell and tissue extracts.
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Can the reconstituted protease inhibitor cocktail solution be stored at -20°C and for how long?
1 answer-
The reconstituted protease inhibitor solution may be stored at 2-8 °C for at least 2 weeks. Long term solution stability studies at -20°C have not been performed, however 10X solutions - 10mL per tablet - will be stable for at least 1 month in frozen aliquots. Avoid repeated freeze/thaw cycles.
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Are there publications that cite use of Product S8830, SIGMAFAST™ Protease Inhibitor Cocktail Tablets?
1 answer-
Yes. Several examples of published papers that cite the use of Product S8830 include these:1. Smith, E.R., et al., Instability of fibroblast growth factor-23 (FGF-23): Implications for clinical studies. Clinica Chimica Acta, 412(11-12), 1008-1011 (2011). Use was with blood at 20 μL cocktail per 1 mL of blood.2. Reber, S., "Isolation of Centrosomes from Cultured Cells" (Chapter 8), from Microtubule Dynamics: Methods in Molecular Biology (Springer Protocols), vol. 777, pp. 107-116 (2011).
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How should I dissolve Product No. S8830, SIGMAFAST™ Protease Inhibitor?
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You can dissolve the product in 100 ml of water to make a 1X solution. You can make a 10X stock solution using 10 ml of water and then dilute 1:10 for use. Concentrations greater than 1X may appear slightly hazy. This will not affect the performance of the protease inhibitors.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?
1 answer-
This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..
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What is the difference between Protease Inhibitors P8849 and S8830?
1 answer-
P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).
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