Key Documents

View All Documentation

α-Amylase from Aspergillus oryzae

Name: α-Amylase from <I>Aspergillus oryzae</I>
Brand: SIGMA

≥150 units/mg protein (biuret)

Synonym(s):

1,4-α-D-Glucan-glucanohydrolase

Select a Size

Change View

Pack Size	SKU	Availability	Price

About This Item

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

232-588-1

NACRES:

NA.54

MDL number:

MFCD00081319

EC Number:

3.2.1.1 (BRENDA, IUBMB)

Specific activity:

≥150 units/mg protein (biuret)

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

form

powder

Quality Level

200

specific activity

≥150 units/mg protein (biuret)

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

Enabling,

storage temp.

−20°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. α-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis [1]. Amylases from Aspergillus oryzae are commonly used as baking additives to prevent staling in the baking industry, clarify haze from fruit juices and alcoholic beverages, and to produce glucose and maltose syrup products [2].

Biochem/physiol Actions

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. The International Union of Immunological Societies (IUIS) has classified α-amylase from Aspergillus oryzae (Asp o II) as an occupational allergen that can cause respiratory symptoms [2].

Physical form

powder

Analysis Note

Crude

Other Notes

One unit will liberate 1.0mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	86250	A8220	10065
Sigma-Aldrich A9857 α-Amylase from Aspergillus oryzae	Sigma-Aldrich 86250 α-Amylase from Aspergillus oryzae	Sigma-Aldrich A8220 α-Amylase from Aspergillus oryzae	Sigma-Aldrich 10065 α-Amylase from Aspergillus oryzae
specific activity ≥150 units/mg protein (biuret)	specific activity ~1.5 U/mg (~0.2 U acc. to Willstätter)	specific activity ≥800 FAU/g	specific activity ~30 U/mg
form powder	form powder	form aqueous solution	form powder
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 100
greener alternative category Enabling,	greener alternative category Enabling,	greener alternative category Enabling,	greener alternative category -
sustainability Greener Alternative Product	sustainability Greener Alternative Product	sustainability Greener Alternative Product	sustainability -

Still not finding the right product?

Explore all of our products under α-Amylase from Aspergillus oryzae

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H302,H334

pcodes

P261 - P264 - P270 - P284 - P301 + P312 - P304 + P340 + P312

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Isolation and denomination of an important allergen in baking additives: alpha-amylase from Aspergillus oryzae (Asp o II).

X Baur et al.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 24(5), 465-470 (1994-05-01)

The commercially available alpha-amylase from Aspergillus oryzae which is widely used as a baking additive was compared with a highly purified enzyme preparation. We used enzyme allergosorbent test (EAST), EAST inhibition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focussing, immunoblotting

Mildly Pasteurized Whey Protein Promotes Gut Tolerance in Immature Piglets Compared with Extensively Heated Whey Protein.

Marit Navis et al.

Nutrients, 12(11) (2020-11-08)

Human milk is the optimal diet for infant development, but infant milk formula (IMF) must be available as an alternative. To develop high-quality IMF, bovine milk processing is required to ensure microbial safety and to obtain a protein composition that

Simultaneous Determination of Total Vitamins B1, B2, B3, and B6 in Infant Formula and Related Nutritionals by Enzymatic Digestion and LC-MS/MS-A Multi-Laboratory Testing Study Final Action: AOAC Method 2015.14.

Sean McClure

Journal of AOAC International, 103(4), 1060-1072 (2020-11-27)

A multi-laboratory study was completed for AOAC First Action Method 2015.14. Ten laboratories from eight countries participated. Each laboratory analyzed (in duplicate) a placebo and 14 fortified nutritionals. Product matrices analyzed included milk, soy, partially hydrolyzed milk, partially hydrolyzed soy

View All Related Papers

Global Trade Item Number

SKU	GTIN
A9857-5MU	04061835553761
A9857-1MU	04061833412589
A9857-250KU	04061833412596

Questions

2 years ago

Please specify quantity for 250KU product no A9857

1 answer
1. Technical Support
  
  2 years ago
  
  This product is sold by activity. The minimum activity specification for this material is 150 units per milligram. The weight of this product can be determined using the activity value reported on the lot-specific Certificate of Analysis(COA). For example, the sample COA has an activity value of 280 units/mg. The amount of powder in the 250,000-unit package will be 892.85mg.
  Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a sample or lot-specific Certificate:
  https://www.sigmaaldrich.com/product/sigma/a9857#product-documentation
  
  Helpful?

Reviews

No rating value

Key Documents

Skip To

α-Amylase from Aspergillus oryzae

≥150 units/mg protein (biuret)

Select a Size

About This Item

form

Quality Level

specific activity

greener alternative product characteristics

sustainability

greener alternative category

storage temp.

General description

Application

Biochem/physiol Actions

Physical form

Analysis Note

Other Notes

Compare Similar Items

This Item

Still not finding the right product?

Safety Information

pictograms

signalword

hcodes

pcodes

Hazard Classifications

Storage Class

wgk

flash_point_f

flash_point_c

ppe

Documentation

Certificates of Analysis (COA)

Already Own This Product?

Peer Reviewed Papers

Global Trade Item Number

Questions

seema.phadtare

Please specify quantity for 250KU product no A9857

Reviews

Active Filters