The recombinant streptavidin from this product is the natural protein and does not contain a polyhistidine tag.
S0677
Streptavidin from Streptomyces avidinii
recombinant, expressed in E. coli, lyophilized powder
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About This Item
Form:
lyophilized powder
Recombinant:
expressed in E. coli
Pricing and availability is not currently available.
Quality Segment
recombinant
expressed in E. coli
form
lyophilized powder
capacity
4 mol/mol binding capacity (Biotin)
storage temp.
−20°C
General description
Streptavidin is a biotin-binding protein that is obtained from Streptomyces avidinii. It is resistant to extreme temperature, pH, detergents and proteolytic enzymes. Streptavidin has a molecular weight of 60kDa and exists as a homotetramer.
Application
Streptavidin from Streptomyces avidinii has been used-
- for blocking of the AFM (atomic force microscopy) tip, during the measurement of unbinding forces in the avidin-biotin system[1]
- for coupling of anti-HA (hemagglutinin) antibodies during cell free synthesis and assembly of proteins on a biochip
- for the coating of the gelatin flow phantoms to eventually determine the feasibility of IVUS (intravascular ultrasound) transducer for (biotinylated) microbubble-based drug delivery
- to immobilize proteins and oligonucleotides
Biochem/physiol Actions
Streptavidin is an antibiotic that functions by binding to and depleting the essential vitamin biotin from the surrounding environment. Because of its unique properties, streptavidin has found various applications in biological studies, including immunotherapy, immunoassays, hybridization assays, lymphocyte activation, antigen localization and affinity chromatography.
Physical form
Lyophilized from 5 mM potassium phosphate buffer
Other Notes
One unit will bind 1.0 μg biotin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| form lyophilized powder | form powder | form - | form essentially salt-free, lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200, 400 | Quality Level 200 |
| capacity 4 mol/mol binding capacity (Biotin) | capacity - | capacity - | capacity 4 mol/mol (Biotin) |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| S0677-25MG | 04061836830533 |
| S0677-1MG | 04061836830519 |
| S0677-5MG | 04061836830540 |
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I am planning to purchase Streptavidin from Streptomyces avidinii (S0677). I would like to know if this protein has a polyhistidine tag on it.
1 answer-
Helpful?
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what is the molecular weight ?
1 answer-
The molecular weight ranges between 55-60 kDa.
Helpful?
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