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85878

Streptavidin from Streptomyces avidinii

affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Form:
powder
Mol wt:
Mr ~60000
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form

powder

Quality Segment

quality

affinity purified, lyophilized from 10 mM potassium phosphate

specific activity

≥13 U/mg protein

mol wt

Mr ~60000

solubility

H2O: 1 mg/mL, clear to hazy, colorless to faintly yellow

storage temp.

−20°C

General description

Streptavidin is a crystalline protein synthesized by Streptomycetes.[1]

Application

Streptavidin from Streptomyces avidinii has been used:
  • in the pre-functionalization of self-propelled catalytic micromotors
  • in the fabrication and modification of hydroxyapatite-chitosan (HA-CTS) nanofilm-coated substrates
  • as an analyte to bind biomolecules to the gold-inverted polymer solar cells (Au-IPSC)

Biochem/physiol Actions

Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin.[1] The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.
The sales quantitites 1 mg and 5 mg

Analysis Note

Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied.

Other Notes

1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5
Review: Application of the avidin-biotin technique in microbiology; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin[2]; Avidin-Biotin technology

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1 of 1

This Item
S0677S9415S4762
form

powder

form

lyophilized powder

form

-

form

essentially salt-free, lyophilized powder

mol wt

Mr ~60000

mol wt

-

mol wt

-

mol wt

~60 kDa

solubility

H2O: 1 mg/mL, clear to hazy, colorless to faintly yellow

solubility

-

solubility

-

solubility

-

specific activity

≥13 U/mg protein

specific activity

-

specific activity

-

specific activity

≥13 units/mg protein

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200, 400

Quality Level

200


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–2 of 2 Questions  
  1. What is the atomic mass of this Streptavidin? Is it 66 kDa?

    1 answer
    1. The atomic mass of this product is not characterized for the finished goods. However, according to the following publication, the apparent molecular weight under SDS-PAGE analysis seems to be about 66 kDa. The reference publication is AIMS Biophysics, 7(4): 236–247.

      Helpful?

  2. Is this a recombinant or native protein?

    1 answer
    1. This product is native streptavidin that has been affinity purified from fermentation cultures of Streptomyces avidinii.

      Helpful?

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