The integrity of Spermidine can be verified by Gas chromatography. The following publication may be of interest for protocol reference on Gas chromatography analysis of Spermidine.
1. J Mass Spectrom. 2009 Aug;44(8):1203-10. doi: 10.1002/jms.1597.
2. A quantitative GC-MS method for three major polyamines in postmortem brain cortex, PMID: 19514045
S0266
Spermidine
BioReagent, Molecular Biology, suitable for cell culture, ≥98%
Synonym(s):
1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane
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About This Item
Linear Formula:
NH2(CH2)3NH(CH2)4NH2
CAS Number:
Molecular Weight:
145.25
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
EC Number:
204-689-0
Beilstein/REAXYS Number:
1698591
MDL number:
Pricing and availability is not currently available.
grade
Molecular Biology
Quality Level
product line
BioReagent
assay
≥98%
form
solid or liquid
mol wt
145.25 (AMU)
technique(s)
cell culture | mammalian: suitable
refractive index
n20/D 1.479 (lit.)
density
0.925 g/mL at 25 °C (lit.)
foreign activity
DNase, RNase, and protease, none detected
storage temp.
room temp
SMILES string
NCCCCNCCCN
InChI
1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
InChI key
ATHGHQPFGPMSJY-UHFFFAOYSA-N
Gene Information
rat ... Ppm1a(24666)
General description
Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.
Application
Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.[1][2]
Spermidine has been used in the preparation of gold beads and as a component in nuclei isolation buffer (NIB).[3]
Biochem/physiol Actions
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T4 polynucleotide kinase activity.
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.
Spermidine helps to maintain the proper activity of gene expression. This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300). Spermidine participates in cell growth.
Disclaimer
Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.
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This Item | |||
|---|---|---|---|
| assay ≥98% | assay ≥99.5% (GC) | assay ≥99% (GC) | assay ≥97.5% (NT), ≥99.0% (GC) |
| form solid or liquid | form - | form solid or liquid (clear) | form - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| technique(s) cell culture | mammalian: suitable | technique(s) ChIP: suitable, buffer exchange: suitable | technique(s) - | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| mol wt 145.25 (AMU) | mol wt - | mol wt - | mol wt - |
| storage temp. room temp | storage temp. - | storage temp. room temp | storage temp. 2-8°C |
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signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
233.6 °F - closed cup
flash_point_c
112 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Elumalai Sivamani et al.
Plant cell reports, 28(2), 213-221 (2008-11-19)
We have developed a method by which remarkably higher efficiencies of transient and stable transformation were achieved in bombardment transformation of plants. Over fivefold increase in transient gus gene expression was achieved when rice or maize suspension cells were bombarded
Spermidine delays aging in humans
Madeo F, et al.
Aging (Albany. NY.), 10(8), 2209-2209 (2018)
Organization, developmental dynamics, and evolution of plastid nucleoids
Sato N, et al.
International Review of Cytology, 232, 217-262 (2003)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S0266-25G | 04061836830175 |
| S0266-1G | 04061836830168 |
| S0266-5G | 04061837898983 |
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Can the reconstituted S0266 solution, which has been sitting at room temperature for 3 months, still be used in CUT&Tag assays to stabilize nuclei? Also, how can the integrity of spermidine be tested? If there's no more spermidine available to test the solution, should more be purchased?
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What is the solvent that spermidine is dissolved in?
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This product is a neat or pure liquid and has not been diluted in a solvent. However, to further dilute this product, it is recommended to use water at 50 mg/mL water.
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How to store S0266-5G? which comes as liquid and what is the molaritty of it? which solvent to use to dilute it?
1 answer-
This product can be stored in room temperature in its original format. The concentration of this product can be determined using the lot specific purity reported on the Certificate of Analysis, the density, and the compound molecular weight. This product has a density of 0.925 g/mL and a molecular weight of 145.25 g/mol. The minimum purity is 98%, so the molarity will vary slightly from lot to lot. The molarity can range from 6.2M (98%) to 6.4M (100%).
Molarity=Density x 1000 x Purity/MWThis product is supplied as a liquid or solid. A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter.
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How to store S0266-1G which comes as liquid? alliquoted and store at -20C? How long it can be stored at this condition?
1 answer-
Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.
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How frequently should new solutions be made?
1 answer-
A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter. Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is this a spermidine solution or a neat liquid, and what is the concentration?
1 answer-
Based on a density of 0.925 g/mL and a molecular weight of 145.25, this neat liquid has an effective molarity of 6.4 M.
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