R1158

Ribonuclease Inhibitor

recombinant, expressed in E. coli, 20-40 units/μL

• Synonym(s): RNAse inhibitor
• UNSPSC Code: 12352200
• NACRES: NA.52
• MDL number: MFCD00132187

Properties

• recombinant: expressed in E. coli
• Quality Segment: 300
• grade: Molecular Biology
• form: buffered aqueous solution
• mol wt: ~50 kDa
• concentration: 20-40 units/μL
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).

Application

Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:

• cDNA synthesis from mRNA
• reverse transcriptase polymerase chain reaction (RT-PCR)
• in vitro transcription and translation
• mRNA protection from degradation
• formaldehyde cross-linked lncRNA purification
• RNA-fluorescence in situ hybridization (FISH)[1]

Biochem/physiol Actions

Inhibits RNase by forming a tight, non-covalent 1:1 complex.

Physical form

Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.

Other Notes

• Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
• This product is for R&D use only, not for drug, household, or other uses.

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable