The exact temperature range for RNase inhibition has not been determined. This product has shown activity from room temperature to 50 °C. Denaturing conditions, such as urea or temperatures above 50 °C, should be avoided as they may cause the release of active ribonuclease from the complex.
R2520
Ribonuclease inhibitor human
Inhibits ribonucleases during transcription and translation experiments
Synonym(s):
RNase Inhibitor, Ribonuclease Inhibitor
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About This Item
Pricing and availability is not currently available.
biological source
human placenta
Quality Segment
grade
Molecular Biology
form
buffered aqueous solution
mol wt
~50 kDa
concentration
30,000-50,000 units/mL
shipped in
dry ice
storage temp.
−20°C
General description
Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is human placenta. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes.
Application
Suitable for use in
- in vitro inhibition of ribonucleases
- cDNA synthesis
- RT-PCR
- in vitro transcription and translation
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
Biochem/physiol Actions
Inhibits RNase by forming a tight, non-covalent 1:1 complex.
Inhibits RNase by forming a tight, non-covalent 1:1 complex. Also inhibits angiogenin, a neovascularization promoter which is homologous with RNase A.
Physical form
Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT
Other Notes
One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.
Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.
Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT
This inhibitor of RNase activity is isolated from human placenta and supplied as a buffered aqueous glycerol solution.
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This Item | |||
|---|---|---|---|
| biological source human placenta | biological source - | biological source - | biological source bovine pancreas |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| form buffered aqueous solution | form buffered aqueous solution | form lyophilized | form lyophilized powder |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~50 kDa | mol wt ~50 kDa | mol wt 13.7 kDa, ~13,700 | mol wt ~13,700 |
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Hi, what is the temperature range in which this product inhibits RNase activity? Thanks,
1 answer-
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