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Phenol solution

Name: Phenol solution
Brand: SIGMA

Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology

Synonym(s):

nucleic acid extraction

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About This Item

Empirical Formula (Hill Notation):

C₆H₆O

CAS Number:

108-95-2

Molecular Weight:

94.11

UNSPSC Code:

12352104

NACRES:

NA.52

PubChem Substance ID:

57654523

MDL number:

MFCD00002143

Beilstein/REAXYS Number:

6428463

Grade:

Molecular Biology

Technique(s):

RNA purification: suitable

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Properties

grade

Molecular Biology

Quality Level

200

product line

BioReagent

form

liquid

technique(s)

RNA purification: suitable

pH

4.1-4.5 (25 °C)

storage temp.

2-8°C

SMILES string

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Gene Information

human ... GABRA1(2554)

Description

General description

Phenol solution is a clear liquid saturated with citrate buffer. Phenols are organic compounds that feature a hydroxyl group directly bonded to an aromatic carbocyclic ring. The common name for monohydroxybenzene is phenol.

Application

Phenol solution has been used:

for the extraction of RNA by standard phenol-chloroform protocols
in cell wall monosaccharide analysis to treat epidermal strips from the abaxial side of Commelina communisin leaves[1]
as a component of lysis-denaturation buffer for RNA isolation

Biochem/physiol Actions

Phenol solution can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol-chloroform protocols. This organic solvent also serves as a potential protein denaturant. Phenol-chloroform extraction is a broadly used method for the extraction of nucleic acids. Despite phenol′s incomplete inhibition of RNase activity, it possesses characteristics that make it useful for isolating nucleic acids when combined with chloroform and alcohol.

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1 of 1

This Item	P4557	48688	40063
Sigma-Aldrich P4682 Phenol solution	Sigma-Aldrich P4557 Phenol solution	Supelco 48688 Phenol solution	Supelco 40063 Phenol solution
technique(s) RNA purification: suitable	technique(s) -	technique(s) HPLC: suitable, gas chromatography (GC): suitable	technique(s) HPLC: suitable, gas chromatography (GC): suitable
grade Molecular Biology	grade Molecular Biology	grade certified reference material, TraceCERT^®	grade certified reference material, TraceCERT^®
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 300
form liquid	form liquid	form -	form -
pH 4.1-4.5 (25 °C)	pH 7.7-8.1 (25 °C)	pH -	pH -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-30°C	storage temp. 2-8°C

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pictograms

GHS06,GHS08,GHS05,GHS09

signalword

Danger

hcodes

H301 + H311 + H331,H314,H341,H373,H411

pcodes

P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

target_organs

Nervous system,Kidney,Liver,Skin

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Effect of ArcA and FNR on the expression of genes related to the oxygen regulation and the glycolysis pathway in Escherichia coli under microaerobic growth conditions.

Sagit Shalel-Levanon et al.

Biotechnology and bioengineering, 92(2), 147-159 (2005-07-01)

Escherichia coli has several elaborate sensing mechanisms for response to the availability of oxygen and the presence of other electron acceptors. The adaptive responses are coordinated by a group of global regulators, which include the one-component Fnr protein, and the

PHENOLS

Smith PWG and Tatchell AR

Aromatic Chemistry, 79-104 (1969)

Effect of oxygen, and ArcA and FNR regulators on the expression of genes related to the electron transfer chain and the TCA cycle in Escherichia coli

Levanon SS, et al.

Metabolic engineering, 7(5-6), 364-374 (2005)

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Global Trade Item Number

SKU	GTIN
P4682-100ML	04061837898815
P4682-400ML	04061837898839

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