L8258
Lectin from Wisteria floribunda
lyophilized powder
Synonym(s):
Wisteria floribunda agglutinin, WFA
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About This Item
Form:
lyophilized powder
Pricing and availability is not currently available.
form
lyophilized powder
Quality Segment
potency
<16 μg/mL agglutination activity
composition
Protein, ~95% E1%/280
storage temp.
−20°C
General description
Application
Lectin from Wisteria floribunda has been used:
- to study the perineuronal nets and parvalbumin nets in mouse brain by immunofluorescence[3]
- to evaluate the amount of N-acetylgalactosamine (GalNAc) transferred to Gaussia luciferase (GLuc) substrates by microplate assay[4]
- to study its binding effects on perineuronal nets[5]
Biochem/physiol Actions
Lectin binds with high affinity to the glycans of polysaccharides, glycolipids, and glycoproteins.[1] It is involved in plant defense.[2]
WFA is not blood group specific, but has an affinity for N-acetyl-D-galactosamine.
Packaging
Package size based on protein content
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.
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This Item | |||
|---|---|---|---|
| form lyophilized powder | form buffered aqueous solution | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| potency <16 μg/mL agglutination activity | potency - | potency ≤0.3 μg/mL mitogenic activity | potency <50 μg per mL agglutination activity (using human blood group B erythrocytes) |
| composition Protein, ~95% E1%/280 | composition - | composition - | composition Protein, ≥70% Lowry (, balance sodium citrate) |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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