This product is soluble in 0.9% sodium chloride solution at a concentration of 1 mg/mL. It is also quality controlled to be soluble in phosphate buffered saline at 1 mg/mL.
L9379
Lectin from Phytolacca americana (pokeweed)
lyophilized powder
Synonym(s):
PWM, Pokeweed mitogen
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About This Item
Form:
lyophilized powder
Pricing and availability is not currently available.
sterility
aseptically filled
Quality Segment
form
lyophilized powder
potency
≤0.3 μg/mL mitogenic activity
storage temp.
2-8°C
General description
Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.
Application
Lectin from Phytolacca americana (pokeweed) has been used:
- as a medium supplement for mononuclear cell incubation, to induce lymphocyte proliferation
- as a positive control in the study of the role of chemokine (C–C motif) ligand 2 (CCL2) in proliferation and apoptosis of decidual leukocytes
- as a supplement in the RPMI (Roswell park memorial institute) 1640 medium for peripheral blood lymphocyte cultures
Biochem/physiol Actions
Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.
Physical form
Partially purified TCA precipitate.
Essentially salt-free.
Essentially salt-free.
Analysis Note
Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.
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This Item | |||
|---|---|---|---|
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| potency ≤0.3 μg/mL mitogenic activity | potency ≤0.3 μg/mL mitogenic activity | potency - | potency <50 μg per mL agglutination activity (using human blood group B erythrocytes) |
| sterility aseptically filled | sterility aseptically filled | sterility - | sterility - |
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Related Content
Product Information Sheet
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L9379-10MG | 04061833966556 |
| L9379-5MG | 04061833966570 |
| L9379-40MG | 04061833966563 |
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Puede disolverse con agua destilada o RPMI?
1 answer-
Helpful?
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Is the product Pokeweed L9379 suitable for cell cultures from B lymphocytes? Or is better to use the product L8777? We culture bone marrow samples from B-cell dyscrasias for cytogenetic studies.
1 answer-
L8777 is recommended for cell culture because it is a sterile product.
Helpful?
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how to prepare Pokeweed to be used as mitogen for B-ALL cytogenetics blood culture
1 answer-
This product can be dissolved in PBS and used at a concentration of up to 3 µg/mL for a mitogenic effect on human peripheral blood mononuclear cells. Concentrations higher than 3 µg/mL may be toxic to these cells.
Helpful?
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