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Lipopolysaccharides from Pseudomonas aeruginosa 10

Name: Lipopolysaccharides from <I>Pseudomonas aeruginosa</I> 10
Brand: SIGMA

purified by trichloroacetic acid extraction

Synonym(s):

LPS

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About This Item

UNSPSC Code:

12352201

NACRES:

NA.25

MDL number:

MFCD00131520

Pricing and availability is not currently available.

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Properties

biological source

Pseudomonas aeruginosa (10)

Quality Level

200

form

lyophilized powder

purified by

trichloroacetic acid extraction

impurities

1-10% Protein (Lowry)

color

white to yellow

solubility

water: 4.90-5.10 mg/mL, hazy to turbid, colorless to light yellow

shipped in

ambient

storage temp.

2-8°C

Description

General description

This product is TCA extracted from Pseudomonas aeruginosa serotype 10. The source strain is ATCC 27316.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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This Item	L9143	L2387	L8643
Sigma-Aldrich L7018 Lipopolysaccharides from Pseudomonas aeruginosa 10	Sigma-Aldrich L9143 Lipopolysaccharides from Pseudomonas aeruginosa 10	Sigma-Aldrich L2387 Lipopolysaccharides from Salmonella typhosa	Sigma-Aldrich L8643 Lipopolysaccharides from Pseudomonas aeruginosa 10
biological source Pseudomonas aeruginosa (10)	biological source Pseudomonas aeruginosa (10)	biological source bacterial (Salmonella typhosa)	biological source Pseudomonas aeruginosa (10)
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
solubility water: 4.90-5.10 mg/mL, hazy to turbid, colorless to light yellow	solubility water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow	solubility water: soluble	solubility water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
color white to yellow	color white to faint yellow	color white to faint yellow	color white to faint yellow

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Pseudomonas aeruginosa pyocyanin modulates mucin glycosylation with sialyl-Lewis(x) to increase binding to airway epithelial cells.

Jayme L Jeffries et al.

Mucosal immunology, 9(4), 1039-1050 (2015-11-12)

Cystic fibrosis (CF) patients battle life-long pulmonary infections with the respiratory pathogen Pseudomonas aeruginosa (PA). An overabundance of mucus in CF airways provides a favorable niche for PA growth. When compared with that of non-CF individuals, mucus of CF airways

Evolutionary conserved mechanisms pervade structure and transcriptional modulation of allograft inflammatory factor-1 from sea anemone Anemonia viridis.

Angela Cuttitta et al.

Fish & shellfish immunology, 67, 86-94 (2017-06-06)

Gene family encoding allograft inflammatory factor-1 (AIF-1) is well conserved among organisms; however, there is limited knowledge in lower organisms. In this study, the first AIF-1 homologue from cnidarians was identified and characterised in the sea anemone Anemonia viridis. The

Effect of dietary replacement of fish meal with insect meal on in vitro bacterial and viral induced gene response in Atlantic salmon (Salmo salar) head kidney leukocytes.

Oda Kvalsvik Stenberg et al.

Fish & shellfish immunology, 91, 223-232 (2019-05-24)

With the fast growth of today's aquaculture industry, the demand for aquafeeds is expanding dramatically. Insects, which are part of the natural diet of salmonids, could represent a sustainable ingredient for aquaculture feed. The aim of the current study was

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Global Trade Item Number

SKU	GTIN
L7018-100MG	04061833275214
L7018-10MG	04061833959558

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