Skip to Content
Merck
All Photos(1)

Documents

TMS-009

Sigma-Aldrich

Safranin-O Staining Solution

Safranin-O is often used to identify chondrocytes that have been derived from both human and rodent mesenchymal stem cells (MSCs).

Synonym(s):

Basic Red 2

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

100

concentration

0.1 % (w/v) in distilled water

technique(s)

cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

General description

Safranin-O, also known as basic red 2, is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring all cell nuclei red. It can also be used for the detection of cartilage, mucin and mast cell granules. Safranin-O is often used to identify chondrocytes that have been derived from both human and rodent mesenchymal stem cells.

Application

Research Category
Stem Cell Research
Research Sub Category
Osteobiology

Arthritis

Quality

Solution is red with no particulates present. Solution is suitable for endpoint cell culture assays.

Physical form

Product is presented as a 0.1% w/v solution diluted in distilled water.

Storage and Stability

Store at room temperature for up to 4 months from date of receipt. If Safraninin-O solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 2

1 of 2

Alizarin-Red Staining Solution Alizarin red is a commonly used stain to identify calcium containing osteocytes in differentiated culture of both human and rodent mesenchymal stem cells (MSCs).

Sigma-Aldrich

TMS-008

Alizarin-Red Staining Solution

Oil Red O solution 0.5% in isopropanol

Sigma-Aldrich

O1391

Oil Red O solution

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

See All

Protocols

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service