Product 84120-25g undergoes testing for a color indication. The quality control testing ensures that the solutions exhibit certain colors at pH 0.3 and 1.0. If the color is correct at these pH ranges, the product passes performance testing. Additionally, other tests are performed. For staining starch granules from algae or other sources, product S8884 is suggested. This product is sent to the Biological Stain Commission for testing, which includes using the dye to ensure chromosomes and bacteria are properly stained.
84120
Safranin O
suitable for microscopy
Synonym(s):
3,7-Diamino-2,8-dimethyl-5-phenylphenazin-5-ium chloride, Safranin, Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
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About This Item
Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
Molecular Weight:
350.84
EC Number:
207-518-8
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099
Pricing and availability is not currently available.
form
powder or crystals
Quality Segment
technique(s)
titration: suitable
pH
10 (20 °C, 10 g/L)
solubility
methanol: 0.01 g/10 mL, red
εmax
≥1000 at 525-535 nm in 50% ethanol
suitability
suitable for microscopy
application(s)
diagnostic assay manufacturing
hematology
histology
microbiology
storage temp.
room temp
SMILES string
[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
InChI
1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
InChI key
OARRHUQTFTUEOS-UHFFFAOYSA-N
General description
Safranin O, also known as Basic Red 2, is a basic, cationic, and metachromatic azine dye used as a biological stain. The characteristic azine chromophore contains the phenazine, a planar, tricyclic dibenzopyrazine, which consists of two non-planar benzene rings linked by two nitrogen atoms. A delocalized positive charge, an alternation of aromatic and quinonoid structures, and diazotizable amine groups constitute the chromophoric system. c-Methylation makes this dye of moderate overall size. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. The dye is commercially available as a chloride.
Application
Safranin O is widely employed as a microbiological stain, especially as a counterstain in Gram staining to distinguish Gram-positive from Gram-negative micro-organisms, and for staining bacterial spores.
- It is used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin.
- It is used to prepare a staining solution, that when used together with other in vitro diagnostic products from our portfolio dyes target structures (e. g. Gram-positive or Gram-negative bacteria, by fixing, where necessary embedding, staining, counterstaining, mounting) in bacteriological specimen materials (for example: smears of bacteriological material that have been air-dried and heat-fixed like sputum, smears from fine needle aspiration biopsies (FNAB), rinses, imprints, effusions, pus, exsudates, liquid and solid cultures), for diagnostic evaluations.
- It is used to demonstrate glycosaminoglycans in human and animal histological specimens and nuclei.
- It is a component of Benda′s polychrome stain.
- It is a component of various polychromes and lignin stains for botanical staining applications.
- It has also been applied for the demonstration of mast cells in cytospin preparations and staining surgical frozen sections.
Biochem/physiol Actions
In microbiology, Gram staining allows fast differentiation of bacteria as Gram-positive or Gram-negative. The mureine structure of the bacteria cell wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet, an aniline dye. After the treatment with iodine solution (Lugol’s solution), a dye-iodine complex will form. During the decolorizing step, this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria and appears blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye after treatment with the decoloring solution. Gram-negative bacteria will be counterstained with safranin solution and will then appear pink to red.
Features and Benefits
- Color stable
- Reproducible
- Offers sufficient sensitivity to reveal all structural details
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This Item | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable |
| suitability suitable for microscopy | suitability - | suitability suitable for microscopy | suitability - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder or crystals | form powder | form liquid | form powder |
| solubility methanol: 0.01 g/10 mL, red | solubility H2O: 1 mg/mL | solubility water: soluble at 20 °C | solubility water: 1 mg/mL, clear, dark red to very dark red and red purple |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
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Danger
hcodes
Hazard Classifications
Eye Dam. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
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