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U7752

Urease from Canavalia ensiformis (Jack bean)

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About This Item

CAS Number:
9002-13-5
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
MFCD00070858
EC Number:
3.5.1.5 (BRENDA, IUBMB)
Biological source:
Canavalia ensiformis
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biological source

Canavalia ensiformis

Quality Level

200

form

solid

mol wt

hexamer 545 kDa, trimer ~272 kDa

contains

~20% dithiothreitol

suitability

suitable for marker for electrophoresis (non-denaturing PAGE)

storage temp.

−20°C

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria.[1] It belongs to the superfamily of amidohydrolases and phosphotriesterases.[2] Urease is also found in leguminous seeds.[3]

Application

Urease from Canavalia ensiformis (Jack bean) has been used:
  • as a component of the molecular mass standard in electrophoresis[4]
  • as a component of the standard in size-exclusion chromatography[5]
  • as a reference in native-polyacrylamide gel electrophoresis (PAGE)

Biochem/physiol Actions

Urease is an essential enzyme of the nitrogen cycle.[1] It plays a role in degrading urea into ammonia and carbamate.[1][2] Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense.[3]

Other Notes

Contains approx. 1 mg protein

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This Item
U025194280U1500
Urease from Canavalia ensiformis (Jack bean)

Sigma-Aldrich

U7752

Urease from Canavalia ensiformis (Jack bean)

Urease from Canavalia ensiformis (Jack bean) Type C-3, powder, ≥600,000 units/g solid

Sigma-Aldrich

U0251

Urease from Canavalia ensiformis (Jack bean)

Urease from Canavalia ensiformis (Jack bean) powder, ~1 U/mg

Sigma-Aldrich

94280

Urease from Canavalia ensiformis (Jack bean)

Urease from Canavalia ensiformis (Jack bean) Type III, powder, 15,000-50,000 units/g solid

Sigma-Aldrich

U1500

Urease from Canavalia ensiformis (Jack bean)

biological source

Canavalia ensiformis

biological source

Canavalia ensiformis

biological source

-

biological source

Canavalia ensiformis

suitability

suitable for marker for electrophoresis (non-denaturing PAGE)

suitability

-

suitability

-

suitability

-

form

solid

form

powder

form

powder

form

powder

mol wt

hexamer 545 kDa, trimer ~272 kDa

mol wt

~544620 Da

mol wt

Mr ~480000

mol wt

~544620 Da

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

300

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
0000541376
0000541376
SLCQ4404
SLCL6040
SLCJ0541

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Global Trade Item Number

SKUGTIN
U7752-1VL04061837410109

Questions

  1. Do you know the activity in U/mg of this urease preparation?

    1 answer

    1. This product is intended for use as a molecular weight marker in electrophoresis. The activity is not determined. Please see the link below to review other Urease products that offer a unit activity:
      https://www.sigmaaldrich.com/substance/ureasefromcanavaliaensiformisjackbean123459002135

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