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94280

Urease from Canavalia ensiformis (Jack bean)

powder, ~1 U/mg

Synonym(s):

Jack bean urease, Urea amidohydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
EC Number:
Specific activity:
~1 U/mg
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form

powder

Quality Segment

specific activity

~1 U/mg

mol wt

Mr ~480000

storage temp.

−20°C

General description

Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease consists of nickel and is present in archaea, bacteria, unicellular eukaryotes and plants.
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Application

Urease from Canavalia ensiformis (Jack bean) has been used in purification and crystallization studies.
Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes [1]. It may be useful for the detection of pathogens as well as heavy-metal ions.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit [2].

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25 °C.
Application in (selective) hydrolysis/condensations;citation structure.

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This Item
94281U0251U1500
specific activity

~1 U/mg

specific activity

~8 U/mg

specific activity

≥600,000 units/g solid

specific activity

15,000-50,000 units/g solid

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

mol wt

Mr ~480000

mol wt

Mr ~480000

mol wt

~544620 Da

mol wt

~544620 Da

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

300


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pictograms

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signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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