Unfortunately, the purification method for these products are considered proprietary. However, the following reference may be of interest.
Purification of Clostridium perfringens phospholipase C (alpha-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein.
T Takahashi et al.
Biochimica et biophysica acta, 351(1), 155-171 (1974-05-10)
P4039
Phospholipase C Peptide
lyophilized powder, Type XIV, ≥150 units/mg protein
Synonym(s):
Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase
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About This Item
CAS Number:
UNSPSC Code:
41106300
NACRES:
NA.32
EC Number:
232-638-2
MDL number:
Pricing and availability is not currently available.
Product Name
Phospholipase C from Clostridium perfringens (C. welchii), Type XIV, lyophilized powder, ≥150 units/mg protein
biological source
bacterial (Clostridium perfringens)
Quality Level
type
Type XIV
form
lyophilized powder
specific activity
≥150 units/mg protein
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
storage temp.
−20°C
Gene Information
Clostridium perfringens str. 13 ... plc(988262)
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
Application
Phospholipase C from Clostridium perfringens has been used as a positive control in sandwich enzyme linked immunosorbent assay (ELISA) and ELISA.
Biochem/physiol Actions
Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
Physical form
Lyophilized powder in buffered salts
Preparation Note
Chromatographically purified
Other Notes
One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| biological source bacterial (Clostridium perfringens) | biological source bacterial (Clostridium perfringens) | biological source - | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Gene Information Clostridium perfringens str. 13 ... plc(988262) | Gene Information Clostridium perfringens str. 13 ... plc(988262) | Gene Information Bacillus cereus ATCC 10987 ... plC(2749087) | Gene Information - |
| type Type XIV | type Type I | type - | type - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P4039-125UN | 04061833621745 |
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Is it possible for you to disclose the outline of the purification method for P7633 and P4039 (just the name of the method is fine)?
1 answer-
Helpful?
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Are Products P7633 and P4039, Phospholipase C from Clostridium perfringens (C. welchii), prepared from the same strain?
1 answer-
Products P7633 and P4039, Phospholipase C, from Clostridium perfringens (C. welchii) are prepared from the same strain.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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