The recommended storage for this product is at -20°C in working aliquots.
P6621
Phospholipase C from Bacillus cereus
≥200 units/mg protein
Synonym(s):
Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
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form
lyophilized powder
Quality Level
specific activity
≥200 units/mg protein
mol wt
23-27 kDa by SDS-PAGE
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
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solubility
H2O: soluble 1.0 mg/mL, faintly hazy to hazy
greener alternative category
storage temp.
−20°C
Gene Information
Bacillus cereus ATCC 10987 ... plC(2749087)
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General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
Application
Phospholipase C (PLC) is used to study adrenoceptor-mediated transmembrane signaling[1]. It is used to degrade inositol-containing phospholipids[2]. Phospholipase C from Bacillus, product P6621, has been used to study how flhF affects the export of PLC and other secretory virulence factors[3].
The enzyme from Sigma has been used in the digestion of phosphatidylserine fraction of Schistosoma mansoni adult worms.[4]
Biochem/physiol Actions
Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid[2]. PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
Unit Definition
One unit will liberate 1.0 μmole of water soluble organic phosphorus from L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.
Physical form
Lyophilized powder containing approx. 10% protein. Remainder; trehalose, zinc sulfate, and potassium phosphate
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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The present study compares the molecular mechanism by which thrombin, platelet-activating factor, and epinephrine induce platelet activation. Thrombin and platelet-activating factor induce an initial activation of phospholipase C, as measured by formation of 1,2-diacylglycerol and phosphatidic acid, during platelet shape
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Schistosome infections are characterized by prominent T cell hyporesponsiveness during the chronic stage of infection. We found that schistosome-specific phosphatidylserine (PS) activated TLR2 and affected dendritic cells such that mature dendritic cells gained the ability to induce the development of
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