This product is tetracycline negative.
F4135
Suero fetal bovino
USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma
Fetal Bovine Serum
Sinónimos:
Suero HI, Sueros HI, FBS, FCS, Suero, sueros, FBS HI, FCS HI
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Acerca de este artículo
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Servicio técnico
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Permítanos ayudarlebiological source
bovine fetus
Quality Segment
sterility
sterile-filtered
product line
FBS Classic
composition
Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
General description
El suero bovino fetal (FBS) sigue siendo un complemento popular para los medios porque aporta una amplia gama de funciones al cultivo celular. EL FBS suministra nutrientes, factores de crecimiento y fijación y ayuda a la proliferación.[1] También protege a las células del daño oxidativo y la apoptosis.[2] EL FBS bloquea la generación de especies de oxígeno reactivo, que de otro modo no se elimina en los sistemas de medios sin suero (SFM).[3]
Application
El FBS procedente de los Estados Unidos se utiliza en una amplia variedad de aplicaciones de cultivo celular. El FBS proporciona muchos factores no definidos de promoción del crecimiento y de mejora de la supervivencia de las células en cultivo.
El suero fetal bovino se ha utilizado:
El suero fetal bovino se ha utilizado:
- como componente de los medios de Eagle modificado de Dulbecco (DME, DMEM),[4] el medio mínimo esencial de Eagle (EMEM),[5] el medio del Roswell Park Memorial Institute (RPMI-1640), el medio Eagle α-modificado (αMEM) (MEM)[6] y el medio Leibovitz L-15
- como componente del medio de bloqueo para el tratamiento de cortes del tronco encefálico[7]
- para mejorar la fijación de explantes dérmicos
Preparation Note
Este producto se prepara mediante calentamiento a 56°C durante 30 minutos. El calentamiento del suero a 56°C durante 30 minutos inactiva el sistema del complemento para inmunoanálisis. La activación por calor también inactiva otros inhibidores indeterminados del crecimiento celular en cultivo.
Preparado mediante calentamiento a 56°C durante 30 minutos.
Analysis Note
- Analizada la presencia de endotoxinas y hemoglobina
- Se ha estudiado la presencia de bacterias, virus y micoplasmas
- Filtrado triple con membrana de 0,1 micra bajo condiciones asépticas
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Este artículo | |||
|---|---|---|---|
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| origin USA origin | origin Australia origin | origin New Zealand origin | origin USA origin |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility - |
| technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable, cell culture | insect: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
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Clase de almacenamiento
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Is the FBS F4135 tetracycline negative?
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Is it possible to store the FBS at a temperature of -80°C, which is lower than the recommended storage temperature of -20°C?
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The FBS should be stored at the recommended temperature of -20°C. Storage at -80°C has not been tested, but there are no anticipated issues. Frost Free Freezers should be avoided for storage.
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Recommended storage is listed as -20C; is it safe/ok to store at -80C?
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The recommended long-term storage temperature for FBS is -20C. For the short-term, such as for shipping, packing on dry ice at -40C is recommended. It is not recommended to store the FBS at temperatures lower than -40C. Please see the guide on freezing and thawing FBS and other sera below:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-culture-media-preparation/storing-and-thawing-serumHelpful?
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Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
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Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Why is serum Heat-Inactivated (HI)?
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Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
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Does Sigma-Aldrich offer delipidated bovine serum?
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At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss
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