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C3228

CelLytic MT Cell Lysis Reagent

For mammalian tissues

Sinónimos:

Cell lysis buffer, Cell lysis reagent

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NACRES:
NA.56
UNSPSC Code:
41116134
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Quality Level

form

solution

Application

CelLytic MT Cell Lysis Reagent has been used in the homogenization of hippocampi tissues, quadriceps muscles from mice, primary human trophoblasts (PHT), soleus muscles from mice and organs of Corti (OCs).

Features and Benefits

  • Gentle: Non-denaturing and does not interfere with downstream applications
  • Convenient: Provided ready to use

Analysis Note

It has been tested, but not limited to the following tissues: rat brain, kidney, muscle, heart, liver, spleen and mouse brain, kidney and muscle.

Other Notes

CelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference with protein interactions and biological activity. CelLytic MT is also used for extraction of cell-line proteins. A volume of 20 mL of CelLytic MT is sufficient for 1 gram of tissue.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

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Este artículo
B7435B7310C8740
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

solution

form

solution

form

liquid

form

liquid


Clase de almacenamiento

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Mechanistic Target of Rapamycin Complex 1 Promotes the Expression of Genes Encoding Electron Transport Chain Proteins and Stimulates Oxidative Phosphorylation in Primary Human Trophoblast Cells by Regulating Mitochondrial Biogenesis
Rosario FJ, et al.
Scientific Reports, 9(1), 246-246 (2019)
Terry D Hinds et al.
The Journal of biological chemistry, 291(48), 25179-25191 (2016-10-21)
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SKUGTIN
C3228-50ML04061838445599
C3228-500ML04061838445582

Questions

  1. What is the composition of C3228 - CelLytic™ MT Cell Lysis Reagent?

    1 answer
    1. The CelLytic™ MT Cell Lysis Reagent is composed of a dialyzable mild detergent, bicine, and 150 mM NaCl. While the exact detergent is proprietary, it can be confirmed that it is not SDS or a Triton-based detergent.

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