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BCA1

Kit del ácido bicinconínico para la determinación de proteínas

for 200-1000 μg/ml protein

Sinónimos:

Análisis de proteínas BCA

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NACRES:
NA.32
UNSPSC Code:
12352106
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Quality Level

200

usage

sufficient for ~500 assays

storage temp.

2-8°C

Application

El kit de ácido bicinconínico se ha utilizado para la estimación de proteínas en muestras de proteoma salival, células renales embrionarias humanas 293 y células luteales.

Biochem/physiol Actions

Las proteínas reducen el metal alcalino Cu(II) a Cu(I) de manera dependiente de la concentración. El ácido bicinconínico es un reactivo cromógeno muy específico del Cu(I), formando un complejo morado con una absorbancia máxima a 562 nm. La absorbancia es directamente proporcional a la concentración de proteínas. Esta es una alternativa al reactivo Folin-Ciocalteu para la determinación de proteínas.

Features and Benefits

  • Valoración colorimétrica sencilla y sensible para proteínas
  • Para la disolución de trabajo se utilizan dos reactivos estables
  • Más rápida y fácil que la valoración Lowry de proteínas
  • Compatible con muchos detergentes iónicos y no iónicos
  • Menor variación entre diferentes proteínas que la valoración de unión al colorante de Bradford
  • Adaptable para su uso en placas de micropocillos

Other Notes

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

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Este artículo
QPBCAB9643DUO82047
Bicinchoninic Acid Kit for Protein Determination for 200-1000 μg/ml protein

Sigma-Aldrich

BCA1

Bicinchoninic Acid Kit for Protein Determination

QuantiPro™ BCA Assay Kit for 0.5-30 μg/ml protein

Sigma-Aldrich

QPBCA

QuantiPro™ BCA Assay Kit

Bicinchoninic Acid solution

Supelco

B9643

Bicinchoninic Acid solution

Duolink® In Situ Wash Buffer, Brightfield

Sigma-Aldrich

DUO82047

Duolink® In Situ Wash Buffer, Brightfield

usage

sufficient for ~500 assays

usage

-

usage

-

usage

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

20-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Solo componentes del kit

Referencia del producto
Descripción
  • Bicinchoninic Acid Solution 1 L
  • 4%(w/v) CuSO4 · 5H2O Solution 25 mL
  • Protein Standard Solution 5 x 1

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1

Clase de almacenamiento

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
0000539848
0000539848
0000502790
0000502807
0000502807

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Número de artículo de comercio global

SKUGTIN
BCA1-1KT04061835571055

Questions

1–4 of 4 Questions  

  1. Good morning. Is the "Bicinchoninic Acid Kit for Protein Determination" complete? i.e. does it contain all the necessary reactants? Is there a need to buy plates, wells or other? Thank you.

    1 answer

    1. This kit does require the use of materials that are dependant on the assay format used, but not provided in the kit. These items are as follows:

      • Spectrophotometer capable of accurately measuring absorbance in the 560 nm region
      • 96-well plates, such as Cat. No. CLS9017
      • 96-well plate sealing film: Cat. No. Z369667

      OR

      • Test tubes, 13 × 100 mm: Cat. No. CLS980013
      • 1 mL Disposable Plastic Cuvettes: Cat. No. C5416

      Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/246/793/bca1pis-ms.pdf

      Helpful?

  2. Can the kit be used for measuring the protein content of marine bacteria grown in artificial sea water?

    1 answer

    1. This product has not been tested for use with marine bacteria in artificial sea water. However, the publication below used the Bicinchoninic acid method to measure total protein in planktonic marine bacteria, indicating that this kit may be suitable.
      Determination of Total Protein Content of Bacterial Cells by SYPRO Staining and Flow Cytometry
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC91485/

      Note that there are a number of potential interfering substances that may impact results. Please see the link below to review the product datasheet, including a list of interfering substances beginning on page 4.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/246/793/bca1pis-ms.pdf

      Helpful?

  3. Does this kit rely on naked-eye colorimetric detection or does it require a fluorescent microscope?

    1 answer

    1. The kit is based on colorimetric, not fluorometric, detection. The color intensity should be measured at A562nm

      Helpful?

  4. Can a standard OD plate reader be used for the BCA1-1KT Kit, which offers a choice of assay tubes and 96-well plates as mentioned in the technical description? Additionally, would the Test tubes, 13 × 100 mm, Catalog Number CLS980013 not be needed?

    1 answer

    1. If a standard ELISA plate reader capable of measuring absorbance in the 560nm region is being used, the 96-well format should be suitable. The test tubes are recommended for larger volume analysis with cuvettes, but if the 96-well format is used, they will not be needed for the samples. However, they are also being utilized to make the BSA protein standard curve dilutions. If there is an alternative method for making the serial standard protein dilutions that ensures thorough mixing and utilizes a low-binding surface to prevent protein absorption, such as borosilicate glass and/or polypropylene tubes, that will be suitable as well.

      Helpful?

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