This is a powdered product. The potency in solution is dependent upon the concentration at which it is prepared. The minimum potency specification for this product is 240 µg/mg. The lot specific value will be reported on the Certificate of Analysis. Please see the link below to review a sample Certificate:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A9528-5G0000247193.pdf
A9528
Anfotericina B from Streptomyces sp.
powder, suitable for cell culture, BioXtra
Sinónimos:
Fungizona
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About This Item
Fórmula empírica (notación de Hill):
C47H73NO17
Número de CAS:
Peso molecular:
924.08
Beilstein:
78342
Número MDL:
Código UNSPSC:
12352207
eCl@ss:
34010400
ID de la sustancia en PubChem:
NACRES:
NA.76
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Nombre del producto
Anfotericina B from Streptomyces sp., powder, γ-irradiated, BioXtra, suitable for cell culture
Nivel de calidad
esterilidad
γ-irradiated
Línea del producto
BioXtra
Formulario
powder
técnicas
cell culture | mammalian: suitable
espectro de actividad antibiótica
fungi
yeast
Modo de acción
cell membrane | interferes
temp. de almacenamiento
2-8°C
cadena SMILES
C[C@H]1O[C@@H](O[C@@H]2C[C@@H]3O[C@](O)(C[C@@H](O)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)CC(=O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\2)C[C@H](O)[C@H]3C(O)=O)[C@@H](O)[C@@H](N)[C@@H]1O
InChI
1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
Clave InChI
APKFDSVGJQXUKY-INPOYWNPSA-N
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Descripción general
Chemical structure: polyene
Aplicación
Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.
Acciones bioquímicas o fisiológicas
La anfotericina B se une a los esteroles formando poros en la membrana y provocando la salida de moléculas pequeñas. La máxima eficacia del producto es contra hongos y levaduras.
Envase
Solubilized Amphotericin B is packaged in amber glass bottle.
Componentes
After reconstitution with water, the product will be a suspension of Amphotericin B, containing deoxycholate as the solubilizing agent. The lyophilized formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride.
Precaución
Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.
Nota de preparación
Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution.
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.
It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.
It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).
Otras notas
Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.
The actual solid weight of the packaged product is calculated according to the UV purity.
Solo componentes del kit
Referencia del producto
Descripción
- Amphotericin B ~ 45 %
- Sodium deoxycholate ~ 35 %
- Sodium phosphate balance
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
STOT RE 1
Código de clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
dust mask type N95 (US), Eyeshields, Gloves
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Certificados de análisis (COA)
Lot/Batch Number
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what is the potency of Amphotericin B solubilized solution?
1 answer-
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How much DSMO do I dissolve the 100mg powder in to achieve a stock concentration of 2.5mg/mL?
1 answer-
Amphotericin B is soluble in DMSO at 30-40 mg/mL. Add 100 mg powder to 40 mL DMSO in order to achieve a 2.5 mg/mL concentration.
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What is the shelf life of Product A9528, Amphotericin B solubilized?
1 answer-
Product No. A9528 has a recommended retest date of 5 years from the initial QC release date. This date can be found on the lot-specific Certificate of Analysis.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the amount of Amphotericin B in Product No. A9528?
1 answer-
The package size is the amount of Amphotericin B present in the vial. If weighing the product, utilize the potency value on the lot specific Certificate of Analysis as a correction factor.
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What concentration should Product A9528, Amphotericin B solubilized, be used at?
1 answer-
We recommend to use Product No. A9528 at 5.6 mg solid per Liter. Cell culture tested amphotericin B solubilized preparation is approximately 45% amphotericin B, 35% sodium deoxycholate, with the balance being sodium phosphate. The amphotericin B is at least 80% amphotericin B and 3-5% amphotericin A by HPLC. The exact amount of amphotericin B in the product can be found on the lot specific Certificate of Analysis under Potency.
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