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A5503

Sigma-Aldrich

Albúmina from chicken egg white

lyophilized powder, ≥98% (agarose gel electrophoresis)

Sinónimos:

Albúmina from hen egg white, Ovalbúmina

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About This Item

Número de CAS:
9006-59-1
EC Number:
232-692-7
MDL number:
MFCD00130424
UNSPSC Code:
12352202
eCl@ss:
42010201
NACRES:
NA.25
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biological source

chicken egg white

Quality Level

300

type

Grade V

assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

44287 Da by calculation

purified by

crystallization

technique(s)

bioconjugation: suitable

solubility

H2O: soluble 40 mg/mL

UniProt accession no.

P01012

storage temp.

2-8°C

Gene Information

chicken ... OVAL(396058)

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General description

La albúmina del huevo de gallina, u ovoalbúmina, es el principal constituyente proteico de la clara del huevo. La albúmina del huevo de gallina es una glucoproteína fosforilada. La porción peptídica de la molécula consta de 385 residuos de aminoácidos y tiene un peso molecular de 42,7 kDa.[1]

Application

Glucoproteína fosforilada que es útil como marcador (~45 kDa) para la electroforesis de proteínas. Se ha utilizado para producir un modelo animal del asma.
La ovoalbúmina es útil como marcador (∼45 kDa) para la electroforesis de proteínas. La ovoalbúmina se ha utilizado para producir un modelo animal del asma. La ovoalbúmina se utilizó como medio para incubación celular en el ensayo EliCell para la detección de citocinas liberadas de los eosinófilos.[2]
En estudios llevados a cabo para analizar los efectos de la localización en las inmunizaciones con ADN se utiliza ovoalbúmina en ensayos de anticuerpos.[3]
La ovoalbúmina se ha utilizado como agente de inducción en estudios modelo de dermatitis atópica.

Biochem/physiol Actions

La calorimetría diferencial de barrido indica que la albúmina del huevo de gallina se desnaturaliza a 84 °C.[4] La ovoalbúmina puede utilizarse como una proteína transportadora para conjugarse a péptidos sintéticos que se utilicen como inmunógenos.

Physical form

Esencialmente exenta de sal

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1A

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000417379
0000417380
0000417381
0000384106
0000384106

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J S Boyle et al.
International immunology, 9(12), 1897-1906 (1998-02-18)
We examined the role of the cellular localization of antigen on the immune response after DNA immunization of mice with three forms of ovalbumin (OVA). DNA encoding OVA which was secreted (sOVA) generated 10- to 100-fold higher IgG responses with
Ken Morishima et al.
Communications biology, 3(1), 294-294 (2020-06-10)
Currently, a sample for small-angle scattering (SAS) is usually highly purified and looks monodispersed: The Guinier plot of its SAS intensity shows a fine straight line. However, it could include the slight aggregates which make the experimental SAS profile different
Ana Raquel Maceiras et al.
Nature communications, 8, 15067-15067 (2017-04-22)
Immunization leads to the formation of germinal centres (GCs) that contain both T follicular helper (Tfh) and T follicular regulatory (Tfr) cells. Whether T-cell receptor (TCR) specificity defines the differential functions of Tfh and Tfr cells is unclear. Here we
A D Nisbet et al.
European journal of biochemistry, 115(2), 335-345 (1981-04-01)
The complete amino acid sequence of hen ovalbumin, comprising 385 residues, has been determined. The sequence was deduced from the 17 cyanogen bromide fragments and from peptides derived by digestion with a number of proteolytic enzymes. The molecular weight of
Joseph Khoory et al.
PloS one, 11(1), e0141206-e0141206 (2016-01-20)
Acute, inflammatory conditions associated with dysregulated complement activation are characterized by significant increases in blood concentration of reactive oxygen species (ROS) and ATP. The mechanisms by which these molecules arise are not fully understood. In this study, using luminometric- and
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Artículos

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

Questions

1–10 of 10 Questions  

  1. Upon resuspension, at what temperature should albumin be stored and how long is it stable?

    1 answer

    1. Solutions may be stored refrigerated at 2-8°C. The long term stability of this product in solution has not been determined. However, filter sterilized solutions of human and bovine origin have a shelf life of 2 - 3 years when stored refrigerated. Albumin solutions may also be stored at -20 °C. Avoid repeated freeze/thaw cycles for frozen solutions.

      Helpful?

  2. Is this endotoxin free ovalbumin?

    1 answer

    1. Unfortunately, this product is not tested for endotoxin.

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  3. Is A5503 fluorescent?

    1 answer

    1. This product is pure ovalbumin with a minimum purity specification of 98%. No fluorescent conjugate or probe is present in this material.

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  4. Does A5503 can analyze by western blotting?

    1 answer

    1. This product has not been specifically tested for use in western blot. However, there is no reason to suspect that this albumin would not be suitable.

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  5. What is the solubility of Product No. A5503, Albumin from chicken egg white, in aqueous buffers (e.g. Tris, PBS)?

    1 answer

    1. We have only tested solubility in water. Salts in buffers may make solubility more difficult.

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  6. Is Product No. A5503, Albumin from chicken egg white, suitable for cell culture or injection?

    1 answer

    1. We do not test any of the ovalbumins in cell culture or in vivo applications. Customers would need to perform suitability testing in their own experiments.

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  7. How is Product No. A5503, Albumin from chicken egg white, analyzed for purity?

    1 answer

    1. A5503 is analyzed by agarose gel electrophoresis. We have not done SDS-PAGE.

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  8. Product No. A5503, Albumin from chicken egg white, is to be stored at 2 - 8°C. Does it ship on ice?

    1 answer

    1. A5503 does not require shipping on ice.

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  9. Is Product No. A5503, Albumin from chicken egg white, endotoxin-tested?

    1 answer

    1. A5503 is not endotoxin-tested. None of the ovalbumins are endotoxin-tested.

      Helpful?

  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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