Freeze-thaw cycles may lead to degradation of added phosphatase and protease inhibitors. It is highly recommended to prepare the cocktail just prior to application.
20-188
Tampón de lisis RIPA, X10
100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.
Sinónimos:
lysis buffer
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About This Item
Código UNSPSC:
41116010
eCl@ss:
42029053
NACRES:
NB.22
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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Formulario
liquid
envase
pkg of 100 mL
fabricante / nombre comercial
Upstate®
técnicas
immunoprecipitation (IP): suitable
western blot: suitable
Condiciones de envío
ambient
Descripción general
El tampón para análisis de radioinmunoprecipitación (RIPA) contribuye a la lisis celular y la solubilización de las proteínas de células de mamífero cultivadas en suspensión y adheridas. Además, este tampón ayuda en los estudios de interacciones proteína-proteína relevantes al reducir las interacciones de unión a proteínas inespecíficas para mantener el fondo bajo, al tiempo que permite que se produzcan la mayoría de interacciones específicas.
Aplicación
Forma física
Tris-HCl 0,5 M, pH 7,4, NaCl 1,5 M, ácido desoxicólico al 2,5 %, NP-40 al 10%, EDTA 10 mM.
Almacenamiento y estabilidad
6 meses a temperatura ambiente
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Salvo que se indique lo contrario en nuestro catálogo o en otra documentación de la empresa que acompañe al producto, nuestros productos están indicados sólo para investigación y no deben utilizarse para ningún otro propósito, como, entre otros, usos comerciales no autorizados, diagnóstico in vitro, usos terapéuticos ex vivo o in vivo o cualquier tipo de consumo o aplicación en humanos o animales.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3 - Eye Dam. 1
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Can I prepare 10 ml of 1x RIPA buffer and add phosphatase and protease inhibitor then aliquot it to 1 ml Eppendorf tubes and store at -20 for use?
1 answer-
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Can this lysis extract protein from the Nucleus?
1 answer-
RIPA Buffer enables the extraction of cytoplasmic, membrane, and nuclear proteins. Certain proteins are more soluble in RIPA buffer than others.
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How long can 1x RIPA lysis buffer be stored with or without the protease?
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This product is stable at room temperature for 6 months from date of shipment. Protease and phosphatase inhibitors should be added immediately prior to use.
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I used the 10X RIPA buffer to lyse my cultured cells. How can I do next? Will it affect the protein quantification by using BCA assay kit? Also, will it affect the SDS-PAGE and Western blot?
1 answer-
Protein samples prepared from cell culture lysates using this 1X RIPA buffer are typically compatible with downstream applications such as BCA assay, SDS-PAGE, and Western blotting. The concentrations of salts and detergents present in the 1X RIPA buffer are relatively low, minimizing interference with these techniques.
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Can we store it a 4 degree, if we want to use it after 6 months?
1 answer-
Studies have not been performed to determine if refrigerated storage will extend the shelf life of this product. The end user would have to determine suitability if used after the recommended 6 months. Note that product R0278, a 1X Ripa Buffer, is stored refrigerated and includes a warranty of 1 year from the date of shipment. Please see the link below to review this product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdfHelpful?
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Someone in lab left the 10X RIPA Buffer in room temperature for overnight. Will it affect the quality of the buffer? Could we continue to use it?
1 answer-
This product does not require refrigeration and is stable at room temperature for up to 6 months.
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Do I used distilled water to dilute to 1X?
1 answer-
The 10X RIPA Lysis Buffer should be diluted with nine parts sterile, distilled water.
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Do I have to dilute to 1X for protein isolation?
1 answer-
Yes, RIPA lysis buffer should be diluted to 1X prior to use.
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