This product can be used to remove endotoxins from solutions. A protocol for use and information on regeneration of the resin are provided in the Product Information Sheet.
biological source
synthetic (organic)
Quality Segment
form
aqueous glycerol suspension
extent of labeling
~1 mg per mL
technique(s)
affinity chromatography: suitable
matrix
Cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
200-500 μg/mL binding capacity (lipopolysaccharide)(from Escherichia coli serotype 0128:B12)
suitability
suitable for chromatography
storage temp.
2-8°C
Application
可用于从溶液中去除内毒素
多粘菌素 B-琼脂糖用于蛋白质色谱、亲和色谱和专业树脂。多粘菌素 B-琼脂糖已用于研究牙周炎和牙髓感染，以及肥胖和 2 型糖尿病。
Physical form
悬浮于含有 0.02％ 叠氮化钠的 50％ 甘油中
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此商品 | |||
|---|---|---|---|
| technique(s) affinity chromatography: suitable | technique(s) - | technique(s) - | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| biological source synthetic (organic) | biological source - | biological source - | biological source - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| form aqueous glycerol suspension | form ready-to-use solution | form lyophilized powder | form - |
| suitability suitable for chromatography | suitability suitable for (Fluorescent detection of gram-negative bacteria), suitable for (Microbiome), suitable for microbiology | suitability - | suitability - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. - |
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
相关内容
Product Information Sheet
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P1411-10ML | 04061834354925 |
| P1411-5ML | 04061834354932 |
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What is the application of this polymyxin-B agarose? can we re-use it for several times? And if yes, how can we regenerate it? thanks!
1 answer-
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What polypropylene column should the we use according to the protocol to wash the resin prior to use?
1 answer-
For washing the resin prior to use, the customer can use the following options:
1. C2603 Mini-Columns: These have a capacity of 0.5 mL and an overall height of 1.9 inches.
2. C2728 Mini-Columns: These have a capacity of 2.4 mL and an overall height of 1.5 inches.
The washing procedure using Polymyxin B Agarose (Product No. P1411) can be carried out in a column or in a batch method. For a column, the customer can use disposable mini columns, such as Product Nos. C2603 (0.5 ml) and C2728 (5.0 ml). If a larger column is needed, the customer can use a polypropylene syringe barrel or use the batch method.
In the batch method, the solution is incubated with the polymyxin B agarose for a minimum of 30 minutes with rotation in either a 15 ml or 50 ml polypropylene tube. The tube is then centrifuged, and the supernatant contains the protein of interest with decreased endotoxin that has been bound to the beads and pelleted in the tube.
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What methods can be used to remove endotoxin from a product?
1 answer-
The product is not tested for endotoxin; however, P1411 may be used to strip endotoxin. The P1411 datasheet includes a suggested protocol for this process. The protocol involves washing the resin, equilibrating the column, dissolving the sample in buffer, eluting with endotoxin-free buffer, and collecting the fractions. The collected fractions containing the protein should then be lyophilized. The polymyxin B agarose can be regenerated at least 10 times, and the resin can be stored in 25% ethanol at 4°C
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