This protease is solubilized at 37 °C in a concentration of 0.2 mg/mL in 10 mM sodium acetate/5 mM calcium acetate buffer, pH 7.5. The solution remains stable when refrigerated for 24 hours. No specified time for solubility is available.
P8811
Protease from Streptomyces griseus
powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid
Synonym(s):
Actinase E, Pronase E
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About This Item
CAS Number:
UNSPSC Code:
12352207
EC Number:
232-909-5
NACRES:
NA.75
eCl@ss:
32160410
MDL number:
Form:
powder
Pricing and availability is not currently available.
product line
BioReagent
Quality Segment
form
powder
specific activity
≥3.5 units/mg solid
technique(s)
cell culture | embryo: suitable
impurities
starch, essentially free
storage temp.
−20°C
General description
Completely inactivated by heating above 80 °C for 15-20 minutes.
Application
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Biochem/physiol Actions
A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
Preparation Note
Collected from culture broth of S. griseus.
Analysis Note
Contains approximately 25% calcium acetate.
Other Notes
An unusually non-specific protease.
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | embryo: suitable | technique(s) - | technique(s) - | technique(s) - |
| form powder | form powder | form powder | form lyophilized |
| specific activity ≥3.5 units/mg solid | specific activity ≥3.5 units/mg solid | specific activity - | specific activity ≥70,000 proteolytic units/g dry wt |
| impurities starch, essentially free | impurities - | impurities - | impurities - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P8811-1G | 04061834401834 |
| P8811-100MG | 04061835569311 |
| P8811-5G | 04061835507368 |
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Can you help me to understand how to resuspend it? what is the concentration solubility?
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What is the recommended reconstitution time for this product?
1 answer-
This protease is solubilized at 37 °C in a concentration of 0.2 mg/mL in 10 mM sodium acetate/5 mM calcium acetate buffer, pH 7.5. The solution remains stable when refrigerated for 24 hours. There is no specified time for solubility.
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What is the recommended expirtation date for P8811-100mg Lot #SLCB5965 rec'd 1/11/21?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services
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