537088

Pronase^® Protease, Streptomyces griseus, Nuclease-Free

Widely used broad-spectrum protease mixture

• Synonym(s): Nuclease-Free Protease, Streptomyces griseus, Pronase^® Protease, Pronase^® Protease, Streptomyces griseus
• CAS Number: 9036-06-0
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD00132092
• eCl@ss: 32160410
• Specific activity: ≥70,000 proteolytic units/g dry wt

Properties

• description: RTECS - UK9595000
• form: lyophilized
• specific activity: ≥70,000 proteolytic units/g dry wt
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: 10 mg/mL
• foreign activity: Nuclease, none detected
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

PRONASE^® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.[1]

Application

Pronase^® Protease, Streptomyces griseus, nuclease-free has been used:

• in single-cell preparations for cardiac side population (CSP) analysis[1]
• in aflatoxin plasma biomarker assay[2]
• to permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion[3]

Biochem/physiol Actions

Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.

Preparation Note

Unstable in solution; reconstitute just prior to use.

Other Notes

Note: 1 KU = 1000 units.

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 2