After reconstitution, Endoproteinase Glu-C from Staphylococcus aureus V8 (P2922) should be aliquoted and stored at -20°C for long-term storage. Stock solutions are stable for up to 1 month at -20°C. For short-term storage, solutions are stable for up to 2 days at 4°C. Avoid repeated freeze-thaw cycles.
P2922
Endoproteinase Glu-C from Staphylococcus aureus V8
Type XVII-B, lyophilized powder, 500-1,000 units/mg solid
Synonym(s):
V8 Protease
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
MDL number:
Specific activity:
500-1,000 units/mg solid
Pricing and availability is not currently available.
type
Type XVII-B
Quality Segment
form
lyophilized powder
specific activity
500-1,000 units/mg solid
mol wt
29 kDa
purified by
chromatography
shipped in
wet ice
storage temp.
−20°C
Gene Information
Staphylococcus aureus subsp. aureus MW2 ... sspA(1003044)
Application
Endoproteinase Glu-C from Staphylococcus aureus V8 has been used to digest reduced and alkylated cyclotides to produce linearized fragments.[1]
It is used for selective cleavage of proteins for amino acid sequence determination[2] or peptide mapping.[3]
Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Product P2922 has been used to linearize cyclic peptides in C. ternatea leaf extract.
Biochem/physiol Actions
Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.
Other Notes
One unit will hydrolyze 1 μmole of N-t-Boc-L-glutamic acid α-phenyl ester per min at pH 7.8 at 37 °C.[4] One unit is equivalent to ~0.004 casein digestion unit.
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This Item | |||
|---|---|---|---|
| specific activity 500-1,000 units/mg solid | specific activity - | specific activity ≥10 units/mg protein | specific activity 20 U/mg |
| Gene Information Staphylococcus aureus subsp. aureus MW2 ... sspA(1003044) | Gene Information - | Gene Information - | Gene Information - |
| form lyophilized powder | form lyophilized powder | form lyophilized | form lyophilized (salt-free) |
| shipped in wet ice | shipped in - | shipped in ambient | shipped in - |
| mol wt 29 kDa | mol wt - | mol wt - | mol wt 30 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. - |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P2922-100UN | 04061834365877 |
| P2922-500UN | 04061834365921 |
| P2922-2.5KU | 04061834365907 |
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We reconstituted the 50 µg vial in 1200 µL of water for injection to obtain a concentration of ≥ 20 IU/mL. We used 200 µL, leaving 1000 µL. Should the remaining solution be stored at 2–8 °C, or would aliquoting and freezing at –20 °C be more appropriate?
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