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P6181

Glu-C Protease

Cleaves at glutamic acid residues, suitable for Mass Spectrometry, from Staphylococcus aureus V8

Synonym(s):

V8 Protease

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About This Item

CAS Number:
66676-43-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
200-350-6
MDL number:
MFCD01324998
EC Number:
3.4.21.19 (BRENDA, IUBMB)
eCl@ss:
32160410
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Product Name

Endoproteinase Glu-C from Staphylococcus aureus V8, suitable for protein sequencing, lyophilized powder

grade

Proteomics Grade

Quality Segment

200

form

lyophilized powder

analyte chemical class(es)

amino acids

suitability

suitable for protein sequencing

storage temp.

−20°C

Application

Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for:
  • obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies.[1]
  • the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination.
  • limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase).
  • the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.

Biochem/physiol Actions

Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine endoprotease, which hydrolyzes peptide bonds at the carboxyl side of glutamyl and aspartyl residues. The specificity of Glu-C is dependent upon the buffer and pH employed as well as the structure around the potential cleavage site. In ammonium acetate (pH 4.0) or ammonium bicarbonate (pH 7.8), the enzyme preferentially cleaves glutamyl bonds; whereas, in phosphate buffer (pH 7.8) Glu-C will cleave at either site. Glu-C is reported to be active in the presence of 0.2% SDS (sodium dodecyl sulfate) and in 4.0M urea.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Questions

1–2 of 2 Questions  

  1. Hi, is this enzyme sterile and suitable for cell culture? Thank you.

    1 answer

    1. This endoproteinase is not sterile and has not been tested for suitability in cell culture. It is recommended for use in protein sequencing assays.

      Helpful?

  2. Hi, I was just wondering you can give me the amino acid sequence for this protein. I can't find it on the page.

    1 answer

    1. The product does not need to be sequenced to get released. Unfortunately there is no in-house information about product sequence.

      Helpful?

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