The molecular weight of this product is approximately 28 kDa. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/299/504/p0389dat.pdf
P0389
Penicillinase from Bacillus cereus
lyophilized powder, ≥1500.00 units/mg protein (using benzylpenicillin)
Synonym(s):
β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥1500.00 units/mg protein (using benzylpenicillin)
Biological source:
Bacillus sp. (Bacillus cereus)
Pricing and availability is not currently available.
biological source
Bacillus sp. (Bacillus cereus)
Quality Segment
form
lyophilized powder
specific activity
≥1500.00 units/mg protein (using benzylpenicillin)
composition
Protein, ~10%
technique(s)
activity assay: suitable
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
2-8°C
General description
Penicillinase from Bacillus cereus has been shown to contain three distinct species when purified and examined for homogeneity by sedimentation analysis, amino-terminal analysis, and vertical acrylamide gel electrophoresis. [1]
Application
Used in the production of penicillin.[2] Penicillinase from Bacillus cereus has been used in a comparison study with penicillinase from Bacillus subtillis where the Michaelis constant, shape of the Ph/activity curve and immunological properties were shown to be significantly different.
Penicillinase can be used to reduce levels of penicillin in patients with severe penicillin reactions. [3]
Used in the production of penicillin.
Biochem/physiol Actions
Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.
Physical form
Lyophilized powder containing phosphate and citrate buffer salts
Analysis Note
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
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This Item | |||
|---|---|---|---|
| technique(s) activity assay: suitable | technique(s) activity assay: suitable | technique(s) - | technique(s) - |
| specific activity ≥1500.00 units/mg protein (using benzylpenicillin) | specific activity ~13 U/mg | specific activity - | specific activity - |
| biological source Bacillus sp. (Bacillus cereus) | biological source Bacillus sp. (Bacillus cereus) | biological source - | biological source Bacillus sp. (Bacillus cereus) |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) microbiology | application(s) - |
| form lyophilized powder | form powder | form liquid | form lyophilized |
| shipped in wet ice | shipped in - | shipped in - | shipped in ambient |
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P0389-5X25KU | 04061832758633 |
| P0389-5KU | 04061834291190 |
| P0389-10KU | 04061834291176 |
| P0389-25KU | 04061834291183 |
| P0389-1KU | 04061838446473 |
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Could you tell me the molecular weight of this enzyme (kDa)? P0389-1KU, THANKS.
1 answer-
Helpful?
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I have just received the product P0389-1KU 1000 units. The protocol I'm going to use requires me to use my 10 mg/ml solution. However, I don't understand what your "1000 Units" of product correspond to. Could you please give me more information pls?
1 answer-
The amount of powder in the vial will depend on the activity reported on the lot specific Certificate of Analysis. For example, lot 0000211383 is reported to have an activity of 115 units per milligram. A package of 1000 units would contain 8.7 mg. The 1000 unit package would prepare 870ul of a 10 mg/mL solution. See the link below to review this Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P0389-1KU0000211383.pdfHelpful?
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