61305

Penicillinase from Bacillus cereus

lyophilized, powder, white, ~13 U/mg

• Synonym(s): β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase
• CAS Number: 9073-60-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.5.2.6 (BRENDA, IUBMB)
• MDL number: MFCD00131819
• Specific activity: ~13 U/mg
• Biological source: Bacillus sp. (Bacillus cereus)

Properties

• biological source: Bacillus sp. (Bacillus cereus)
• Quality Segment: 100
• form: powder
• quality: lyophilized
• specific activity: ~13 U/mg
• mol wt: M_r ~30000
• technique(s): activity assay: suitable
• color: white
• suitability: suitable for component for culture media
• application(s): diagnostic assay manufacturing
• foreign activity: β-lactamase II ≤2%
• storage temp.: −20°C

Description

General description

Penicillinase from Bacillus cereus is both an extracellular and cell-bound enzyme. It has a single polypeptide chain and has a molecular weight of 31 kDa. It lacks cysteine in the protein sequence.

Application

Used in the production of penicillin.

Penicillinase from Bacillus cereus may be used in the preparation of biosensor for amperometric detection of hydrolysis of penicillin.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Used to destroy β-lactam antibiotics in body fluids or culture media. For the determination of penicillin; In the coupled enzyme assay for isopenicillin N synthetase; ELISA of estradiol; Penicillinase optodes.

Safety Information

• flash_point_c: Not applicable
• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves