Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
I1146
R3 IGF-1 human
recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
Human IGF-1, IGF-1 Protein, Recombinant IGF-1
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About This Item
NACRES:
NA.77
UNSPSC Code:
51111800
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥85% (HPLC)
Form:
lyophilized powder
Mol wt:
7,676 Da
Pricing and availability is not currently available.
Application
R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.
Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.
Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.
Biochem/physiol Actions
Proliferation of chinese hamster ovary CHO cells.
Preparation Note
Prepared from 0.1 M acetic acid
Other Notes
Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.
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This Item | |||
|---|---|---|---|
| biological source human | biological source human | biological source human | biological source human |
| assay ≥85% (HPLC) | assay ≥95% (SDS-PAGE) | assay ≥95% (HPLC) | assay ≥97% (SDS-PAGE) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| I1146-.1MG | 04061833847503 |
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What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
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How do the sequences of Products I1146 and I1271 compare to that of native Insulin-like Growth Factor 1 (IGF-1)?
1 answer-
The entire IGF-1 gene codes for 194 amino acids, but the mature form of the protein is 70 amino acids, consisting of amino acids 49-118.Product I1146 is R3 IGF-1, which is also 70 amino acids in length. The Glutamic acid (E) at position 3 has simply been replaced by an Arginine (R). So the sequence of Product I1146 is:GP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSAProduct I1271 is the same as I1146, but with an additional thirteen amino acids "in front of" the normal N-terminus of the R3 IGF-1:MFPAM PLSSLFVNGP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSANote that the first 13 amino acids in the recombinant I1271 (MFPAMPLSSLFVN)are quite different from what would have been the preceding 13 amino acids in the immature IGF-1.
Helpful?
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