ROAMYGL

Amyloglucosidase

from Aspergillus niger

• Synonym(s): Glucoamylase, disaccharidase-type-α-D-glucosidase
• UNSPSC Code: 12352204
• EC Number: 3.2.1.3 (BRENDA, IUBMB)
• NACRES: NA.54
• Specific activity: ~14 units/mg protein (At 25 °C with glycogen as the substrate; standardized with BSA.)
• Biological source: Aspergillus niger

Properties

• biological source: Aspergillus niger
• form: suspension
• specific activity: ~14 units/mg protein (At 25 °C with glycogen as the substrate; standardized with BSA.)
• mol wt: M_r 97 kDa
• packaging: pkg of 10 mL (10102857001 [100 mg])
• manufacturer/tradename: Roche
• parameter: 55 °C optimum reaction temp.
• optimum pH: 4.6-4.8
• storage temp.: 2-8°C

Description

General description

Amyloglucosidase is synthesized by several Aspergillus genus species. It is a disaccharidase–type α-glucosidase. This enzyme is an exo-enzyme and one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.

Application

Amyloglucosidase from Aspergillus niger can be used for the hydrolyzation of terminal α1,4- and α1,6-glucosidic bonds (glucose-glucose bonds) in polysaccharides (e.g., starch, dextrins, glycogen), removing glucose units sequentially from the non-reducing end of the molecule. The enzyme will also cleave maltose and maltosides (maltotriose, maltotetraose, etc.).[1]

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides.[2] Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry for the commercial production of D-glucose from corn syrups.[3][3]

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.
Heat inactivation: Heat inactivation is recommended at 80 °C for 45 minutes, followed by rapidly cooling down.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Other Notes

For life science research only. Not for use in diagnostic procedures.

Unit Conversion: One unit (+25 °C; glycogen as substrate) corresponds to 8.6 U (+60 °C; starch as substrate).

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash