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444203

β-Mercaptoethanol, Molecular Biology Grade

A disulfide-reducing agent.

Synonym(s):

2-Mercaptoethanol, BME, β-Mercaptoethanol, 2-Hydroxyethylmercaptan, Thioethylene glycol

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About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
Molecular Weight:
78.13
NACRES:
NA.25
UNSPSC Code:
12161504
Beilstein/REAXYS Number:
773648
MDL number:
Assay:
≥99% (GC)
Grade:
Molecular Biology
Bp:
157 °C (lit.)
Vapor pressure:
1 mmHg ( 20 °C)
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grade

Molecular Biology

Quality Level

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

description

Merck USA index - 14, 5869

assay

≥99% (GC)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

concentration

14.3 M (pure liquid)

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

mp

<-50 °C

density

1.12 g/cm3 at 20 °C, 1.114 g/mL at 25 °C (lit.)

cation traces

heavy metals (as Pb): ≤0.0001%

absorption

≤0.31 at 280 nm, ≤1.50 at 260 nm

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

15-25°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

General description

A disulfide-reducing agent.
A disulfide-reducing agent. Reagent commonly used in organic synthesis (aborsorbance: ≤1.50 at 260 nm; ≤0.31 at 280 nm).

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Toxic (F)

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This Item
M314863689PHR9274
grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

certified reference material, TraceCERT®

assay

≥99% (GC)

assay

99% (GC/titration)

assay

≥99.0% (GC)

assay

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

300

form

liquid

form

liquid

form

liquid

form

-

vapor density

2.69 (vs air)

vapor density

2.69 (vs air)

vapor density

2.69 (vs air)

vapor density

2.69 (vs air)

bp

157 °C (lit.)

bp

157 °C (lit.)

bp

157 °C (lit.)

bp

157 °C (lit.)


signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup



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Global Trade Item Number

SKUGTIN
444203-250ML07790788050368

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. What is the concentration of the solution (in w/v or M)?

    1 answer
    1. The concentration of this product is 14.3 M as a pure liquid.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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