63689

2-Mercaptoethanol

BioUltra, Molecular Biology, ≥99.0% (GC)

• Synonym(s): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
• Linear Formula: HSCH₂CH₂OH
• CAS Number: 60-24-2
• Molecular Weight: 78.13
• UNSPSC Code: 12161504
• NACRES: NA.25
• PubChem Substance ID: 57651953
• EC Number: 200-464-6
• Beilstein/REAXYS Number: 773648
• MDL number: MFCD00004890

Properties

• grade: Molecular Biology
• Quality Level: 200
• vapor density: 2.69 (vs air)
• vapor pressure: 1 mmHg ( 20 °C)
• product line: BioUltra
• assay: ≥99.0% (GC)
• form: liquid
• expl. lim.: 18 %
• reaction suitability: reagent type: reductant
• concentration: 14.3 M (pure liquid)
• technique(s): RNA extraction: suitable, protein extraction: suitable
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
• refractive index: n20/D 1.500 (lit.), n20/D 1.501
• pH: 4.5-6 (20 °C, 500 g/L)
• bp: 157 °C (lit.)
• solubility: H₂O: 0.5 M at 20 °C, clear, colorless
• density: 1.114 g/mL at 25 °C (lit.)
• SMILES string: OCCS
• cation traces: Al: ≤0.5 mg/kg, Ba: ≤0.1 mg/kg, Bi: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Cd: ≤0.05 mg/kg, Co: ≤0.02 mg/kg, Cr: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, K: ≤0.5 mg/kg, Li: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Mo: ≤0.1 mg/kg, Na: ≤1 mg/kg, Ni: ≤0.05 mg/kg, Pb: ≤0.1 mg/kg, Sr: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg
• λ: 0.5 M in H₂O
• UV absorption: λ: 260 nm A_max: 1.5, λ: 280 nm A_max: 0.3
• storage temp.: 2-8°C
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

Application

2-Mercaptoethanol has been used

• in the protein extraction buffer prepared for leaf samples.[1]
• as a supplement in cell culture media.
• in the procedure of RNA extraction.

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds[2]

Safety Information

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• pcodes: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• target_organs: Liver,Heart
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 3
• flash_point_f: 165.2 °F - closed cup
• flash_point_c: 74 °C - closed cup
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter