As provided, this product contains 100 units per vial. The mass weight per vial is not determined.
U3383
Urease Buffer Reagent
Synonym(s):
Urea colorimetric determination assay reagent
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About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Pricing and availability is not currently available.
description
When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.
Quality Segment
form
powder
mol wt
545 kDa
storage temp.
2-8°C
General description
Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia. [1]
Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide.[2][3] It is secreted by several bacterial species, invertebrates, fungi, and plants.[3] Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections.[2] two Ni2+ ions bridged by a hydroxyl group and a carbamylated lysine.[3]
Application
Urease Buffer Reagent has been used:
- to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method[4]
- in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells[5]
- to study the metabolic capacity of rat hepatocytes[6]
Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.
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This Item | |||
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| Quality Level 200 | Quality Level 100 | Quality Level 300 | Quality Level - |
| form powder | form powder | form powder | form - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 545 kDa | mol wt Mr ~480000 | mol wt ~544620 Da | mol wt - |
| description When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative. | description - | description - | description - |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| U3383-1VL | 04061837409486 |
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What's the concentration of Jack bean Urease? I can only see the concentration of buffer.
1 answer-
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How many urease units does 1 vial contain?
1 answer-
As provided, this product contains 100 units per vial.
Helpful?
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What is the volume of water used for reconstitution of 1 vial?
1 answer-
As mentioned on the sample Certificate of Analysis, 30 mL of purified water in one vial of this material yields a clear solution.
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