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S6782

Superdex®

Superdex®200 Prep Grade

Synonym(s):

Size exclusion media, Superdex column

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
MFCD00166816
Pricing and availability is not currently available.
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Quality Segment

200

form

suspension (20% aqueous ethanol)

technique(s)

protein array: suitable

matrix

cross-linked agarose

particle size

24-44 μm

pore size

1-100 kDa MW range (dextrans), 10-600 kDa MW range (proteins)

operating pH range

1-14(short term), 3-12(long term)

storage temp.

2-8°C

General description

Combines the high selectivity of a Sephadex matrix with the chemical and physical stability of crosslinked agarose.

Application

Optimized for high resolution preparative separations by size exclusion chromatography. Recommended fractionation ranges are listed for globular proteins and dextrans.
Superdex® is used for protein chromatography, gel filtration chromatography, gel filtration media, resins and separation media. Superdex® has been used for the purification and characterization of a novel laccase from the edible mushroom Hericium coralloides. Superdex® has also been used for the purification and mechanism exploration of a potential human hepatocellular carcinoma inhibitor from Bauhinia purpurea L. seeds.

Legal Information

Superdex is a registered trademark of Cytiva

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This Item
S6657GE17-1043-01CL4B200
Superdex® Superdex®200 Prep Grade

Sigma-Aldrich

S6782

Superdex®

Superdex® Superdex®75 Prep Grade

Sigma-Aldrich

S6657

Superdex®

Superdex® 200 Prep Grade Cytiva 17-1043-01, pack of 150 mL

GE17-1043-01

Superdex® 200 Prep Grade

Sepharose™ CL-4B Cross-linked

Supelco

CL4B200

Sepharose™ CL-4B

matrix

cross-linked agarose

matrix

cross-linked agarose

matrix

cross-linked agarose, spherical

matrix

Agarose

particle size

24-44 μm

particle size

24-44 μm

particle size

24-44 μm

particle size

-

pore size

1-100 kDa MW range (dextrans), 10-600 kDa MW range (proteins)

pore size

3-70 kDa MW range (proteins), 500-30,000 Da MW range (dextrans)

pore size

-

pore size

30,000-5,000,000 fractionation range (Dextrans), 60,000-20,000,000 fractionation range (Globular proteins)

form

suspension (20% aqueous ethanol)

form

suspension (20% aqueous ethanol)

form

resin

form

beads

technique(s)

protein array: suitable

technique(s)

protein array: suitable

technique(s)

liquid chromatography (LC): suitable

technique(s)

LPLC: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-30°C

storage temp.

2-8°C

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pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

104.0 °F

flash_point_c

40 °C

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW5284
MKCW4326
MKCX0309
MKCQ4003
MKCR2480

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Thomas Ouellette et al.
Protein expression and purification, 30(2), 156-166 (2003-07-26)
A recombinant form of human rhIL-7 was overexpressed in Escherichia coli HMS174 (DE3) pLysS under the control of a T7 promoter. The resulting insoluble inclusion bodies were separated from cellular debris by cross-flow filtration and solubilized by homogenization with 6
J L Van Hove et al.
Biochemistry and molecular biology international, 43(3), 613-623 (1997-11-14)
Large quantities of recombinant acid alpha-glucosidase are needed for in vivo experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A
Mario Jiz et al.
Infection and immunity, 76(7), 3164-3169 (2008-04-23)
Despite effective chemotherapy, schistosomiasis remains a major public health problem in the developing world, with at least 200 million active infections resulting in significant morbidity. Rapid reinfection after treatment, accompanied by extensive residual morbidity, mandates alternative control strategies, including vaccine
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Global Trade Item Number

SKUGTIN
S6782-100ML04061836959593

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