S3887

Streptavidin–β-Galactosidase conjugate

streptavidin 1-2 units/mg protein, lyophilized powder

• Synonym(s): Streptavidin–beta-Galactosidase conjugate
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00132385

Properties

• tag: beta-galactosidase tagged
• Quality Level: 200
• form: lyophilized powder
• streptavidin activity: 1-2 units/mg protein
• composition: Protein, ≥80% biuret
• storage temp.: −20°C

Description

General description

Streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii, is a very high affinity and specificity non-covalent biotin binding protein that is used in a wide range of biochemical/immunological assays such as western blot and ELISAs. It is a component of the streptavidin-biotin system; wherein biotin is conjugated to a target molecule that is later detected by a signal tagged streptavidin. Streptavidin may be covalently tagged with a range of enzymes that develop chromogenic sustrates or fluorescent molecules to support detection systems.
β-galactosidase may be used in genetic, molecular and immunologic detection systems. Active β-galatosidase cleaves the detection molecule X-gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) to form an intense blue product that may be detected by chromogenic techniques.

Application

Streptavidin–β-Galactosidase conjugate may be used in conjunction with biotin labeled molecules in techniques such as ELISA, immunohistochemistry, protein blotting or with biotinylated DNA probes. It provides a convenient yet highly sensitive and specific detection system.
Streptavidin–β-Galactosidase conjugate has been used in the fusion assay in chinese hamster ovary cells.

Biochem/physiol Actions

Streptavidin–β-Galactosidase conjugate is reactive with biotin labeled molecules.

Physical form

Contains Tris buffer salts.

Preparation Note

Streptavidin and β-galactosidase grade VIII are conjugated by a modification of the method of O′Sullivan and purified by gel filtration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)