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S2640

Serum Replacement 3 (50×)

liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Serum Substitute

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About This Item

NACRES:
NA.71
UNSPSC Code:
12352207
Sterility:
sterile-filtered
Form:
liquid
Pricing and availability is not currently available.
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sterility

sterile-filtered

Quality Segment

200

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

General description

Sigma′s Serum Replacement 3 is a fortified, multipurpose serum replacement for the long-term culturing of anchorage dependant and suspension cultures. This serum replacement is effective in research situations such as: studying the effects of growth factors or in the manufacturing of cell derived products.

Application

Serum Replacement 3 (50×) has been used as a supplement in modified phosphate-buffered saline (DPBS) and as a replacement for the growth medium for in vitro experiments for Tendon cells.

Analysis Note

Stable for 30 days when diluted in cell culture medium.

Other Notes

Defined serum replacement designed primarily for the long-term growth of human cells or other mammalian cells. Recommended for use in the production of cell-secreted proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, human recombinant insulin). Does not contain growth factors, steroid hormones, glucocorticoids, cell adhesion factors, detectable Ig and mitogens. May be used with anchorage-dependent and suspension cells.

Disclaimer

This product is tested and found non-reactive for Hepatitis B Surface Antigen (HBsAg) and non-reactive for Human Immunodeficiency Virus (HIV) antibody by ELISA. Nevertheless, products of human origin should be considered potentially infectious and handled accordingly.

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1 of 1

This Item
S9388H1270F7942
Serum Replacement 3 (50×) liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

S2640

Serum Replacement 3 (50×)

Serum Replacement 2 (50×) liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

S9388

Serum Replacement 2 (50×)

Horse Serum Donor herd, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

H1270

Horse Serum

Fetal Bovine Serum Canada origin, sterile-filtered, γ-irradiated, suitable for cell culture

Sigma-Aldrich

F7942

Fetal Bovine Serum

form

liquid

form

liquid

form

liquid

form

-

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

γ-irradiated, sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

≤10 EU/mL Endotoxin

impurities

≤10 EU/mL Endotoxin

Quality Level

200

Quality Level

-

Quality Level

500

Quality Level

500

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKDB5338
MKDB6818
MKCZ6040
MKCZ5703
MKCS2481

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Global Trade Item Number

SKUGTIN
S2640-100ML04061836920098
S2640-10ML04061836920197

Questions

1–2 of 2 Questions  

  1. What is the difference between Serum Replacement 1, 2, and 3?

    1 answer

    1. These concentrated serum replacements are defined, as multipurpose products designed to eliminate the need for serum in culture. These products do not contain growth factors, steroid hormones, or mitogens.

      S0638 Serum Replacement 1, 50X
      Total protein concentration at 1X of 770 mg/mL
      Highly purified bovine serum albumin (heat treated)
      Bovine transferrin (heat treated)
      Bovine insulin

      S9388 Serum Replacement 2, 50X
      Total protein concentration at 1X of 570 mg/mL
      Highly purified bovine serum albumin (heat treated)
      Bovine transferrin (heat treated)
      Bovine insulin

      S2640 Serum Replacement 3, 50X
      Total protein concentration at 1X of 664 mg/mL
      Highly purified human serum albumin (heat treated)
      Human transferrin (heat treated)
      Human (recombinant) insulin

      Helpful?

  2. Does the Serum Replacement 3 contain heat-treated bovine serum albumin, transferrin and insulin, like with Serum Replacement 1 and 2?

    1 answer

    1. As described in the "Other Notes" section of the product detail page, this is a defined serum replacement designed primarily for the long-term growth of human cells or other mammalian cells. Recommended for use in the production of cell-secreted proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, human recombinant insulin). Does not contain growth factors, steroid hormones, glucocorticoids, cell adhesion factors, detectable Ig and mitogens. May be used with anchorage-dependent and suspension cells. It does not contain bovine proteins like products S0638, Serum Replacement 1 (50×) and S9388, Serum Replacement 2 (50×).

      Helpful?

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