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P7348

T7 Phage Promoter Primer Set

Synonym(s):

5′-TAA TAC GAC TCA CTA TAG GG-3′

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About This Item

UNSPSC Code:
41106305
Storage temp.:
2-8°C
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storage temp.

2-8°C

Application

T7 Phage Promoter Primer Set is a single-stranded oligonucleotide with 5′-hydroxyl and 3′-hydroxyl ends and a selection of four fluorescent lables for use in polymerase chain reaction protocols (PCR). Functionally tested for use in fluorescence-detection automated sequencing.

Packaging

Packaged in amber tubes to protect from light.

Analysis Note

Tested for purity with HPLC, PAGE, and OD.

Other Notes

Set of four vials of primer, each individually labeled at the 5′-end with:
FAM: (5[6]-carboxyfluorescein)
JOE: (6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein)
ROX: (5[6]-carboxy-X-rhodamine), and
TAMRA: (5[6]-carboxytetramethylrhodamine.
Supplied as solutions, 50 μl each, in tris-EDTA buffer, pH 7.5, at 1 picomole/μl


Storage Class

10 - Combustible liquids



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Yajing Zhou et al.
PloS one, 6(7), e22224-e22224 (2011-07-27)
Eukaryotic DNA polymerase δ (pol δ) plays a crucial role in chromosomal DNA replication and various DNA repair processes. It is thought to consist of p125, p66 (p68), p50 and p12 subunits. However, rigorous isolation of mammalian pol δ from
High-throughput sequencing of PCR products tagged with universal primers using 454 life sciences systems.
Daigle D, Simen BB, Pochart P.
Current Protocols in Molecular Biology, Unit 7-Unit 7 (2011)
Jacquelina Williams-Woods et al.
Journal of virological methods, 178(1-2), 253-257 (2011-10-04)
Human norovirus (HuNoV) and hepatitis A (HAV) are recognized as leading causes of non-bacterial foodborne associated illnesses in the United States. DNA sequencing is generally considered the standard for accurate viral genotyping in support of epidemiological investigations. Due to the



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