This product is lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2, to which no preservatives have been added. The protein content of each vial is determined by absorbance at 280nm after reconstitution with 1.0 mL of 0.135M sodium chloride. The expected E1% at 280nm is 14.0. It is recommended to reconstitute using the same solvent. Other manufacturers may recommend other solvents and conditions.
M8645
Anti-Mouse IgG (whole molecule) antibody produced in goat
affinity isolated antibody, lyophilized powder
Synonym(s):
Goat Anti-Mouse IgG, Goat IgG Antibody
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ODD
Citations:
15
Pricing and availability is not currently available.
biological source
goat
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
lyophilized powder
technique(s)
Ouchterlony double diffusion: suitable
storage temp.
2-8°C
target post-translational modification
unmodified
General description
IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Immunogen
IgG purified from pooled normal mouse serum.
Application
Anti-mouse IgG (whole molecule) antibody (diluted 1:20) can be used in Indirect immunofluorescence. It can also be used as a secondary antibody in western blotting to release NC1 domain of type IV collagen. Further, it can also be used in enzyme immunoassays (50 μgram) .
Anti-mouse IgG (whole molecule) antibody (diluted 1:20) can be used in:
- indirect immunofluorescence
- western blotting to release NC1 domain of type IV collagen
- enzyme immunoassays (50 μgram)
Biochem/physiol Actions
IgG regulates several functions such as phagocytosis and hence plays a pivotal role in facilitating cytological immune responses. Anti-mouse IgG (whole molecule) antibody can be used in T cell proliferation assays. It can also be used in Ouchterlony Double Diffusion and immunoelectrophoresis. Goat anti- mouse IgG (whole molecule) antibody reacts specifically with mouse IgG subclasses IgG1, IgG2a, IgG2b and IgG3. The product has also shown no reactivity to human serum proteins.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Physical form
Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2
Preparation Note
Reconstitute with 1 mL of 0.135 M sodium chloride.
Other Notes
Antibody adsorbed with human serum proteins
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form whole antiserum | antibody form IgG fraction of antiserum |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source goat | biological source goat | biological source goat | biological source goat |
| technique(s) Ouchterlony double diffusion: suitable | technique(s) Ouchterlony double diffusion: suitable | technique(s) indirect ELISA: 1:70,000, quantitative precipitin assay: 3.2-4.8 mg/mL | technique(s) Ouchterlony double diffusion: 1:8 |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| M8645-5X1MG | 04061833630938 |
| M8645-1MG | 04061834063469 |
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I noticed the reconstitution protocol many other antibodies including anti-mouse IgG from other manufacturers buffer solutions like PBS are most common. Why does this Ab require 0.135M NaCl for reconstitution? What are the benefits of using this solution?
1 answer-
Helpful?
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