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M16 Medium

Name: M16 Medium
Brand: SIGMA

With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

Synonym(s):

Cell Culture Medium, Cell Culture Medium M16, M16 Medium Solution

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

Pricing and availability is not currently available.

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Properties

Quality Level

300

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

NaHCO₃: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

Description

Application

M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

Preparation Note

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Analysis Note

Presence of particulates in solution will not affect product′s performance.

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

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This Item	M7167	R5158	14610C
Sigma-Aldrich M7292 M16 Medium	Sigma-Aldrich M7167 M2 medium	Sigma-Aldrich R5158 RPMI-1640 Medium	SAFC 14610C EX-CELL^® 610-HSF Serum-Free Medium for Hybridoma Cells
technique(s) cell culture \| embryo: suitable	technique(s) cell culture \| embryo: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
form liquid	form liquid	form liquid	form liquid
components NaHCO₃: 2.101 g/L phenol red: 0.0106 g/L sodium pyruvate: 0.0363 g/L glucose: 1.0 g/L (Dextro)	components HEPES: 5.42726 g/L NaHCO₃: 0.35 g/L glucose: 1.0 g/L (Dextro) phenol red: 0.0106 g/L sodium pyruvate: 0.0363 g/L	components phenol red: 0.0053 g/L L-glutamine: 0.3 g/L NaHCO₃: 2 g/L HEPES: 5.96 g/L glucose: 2 g/L (Dextro)	components glucose: 3.45 g/L NaHCO₃: 1.60 g/L L-glutamine: 0.333 g/L HEPES: 7.5 mM
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

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CFP1 coordinates histone H3 lysine-4 trimethylation and meiotic cell cycle progression in mouse oocytes.

Qian-Qian Sha et al.

Nature communications, 9(1), 3477-3477 (2018-08-30)

Trimethylation of histone H3 on lysine-4 (H3K4me3) is associated with gene-regulatory elements, but its transcription-independent function in cell division is unclear. CxxC-finger protein-1 (CFP1) is a major mediator of H3K4 trimethylation in mouse oocytes. Here we report that oocyte-specific knockout

Evaluation of RNA-knockdown strategies for modulation of cytochrome P450 reductase activity in mouse hepatocytes.

Neil Cw Mackenzie et al.

Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research, 6(2), 416-421 (2011-02-26)

Transgenic technologies can provide important animal models for studying drug-metabolizing enzymes. Our overall aim was to generate versatile cell and animal systems that exhibited varying levels of cytochrome P450 oxidoreductase (POR) activity, more accurately modelling the human population for pharmacological

Transcriptome Changes in the Mink Uterus during Blastocyst Dormancy and Reactivation.

Xinyan Cao et al.

International journal of molecular sciences, 20(9) (2019-05-01)

Embryo implantation in the mink follows the pattern of many carnivores, in that preimplantation embryo diapause occurs in every gestation. Details of the gene expression and regulatory networks that terminate embryo diapause remain poorly understood. Illumina RNA-Seq was used to

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Global Trade Item Number

SKU	GTIN
M7292-50ML	04061834061878
M7292-100ML	04061834061854

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