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L8902

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture

Synonym(s):

Phaseolus vulgaris agglutinin, PHA

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About This Item

UNSPSC Code:
12352202
EC Number:
232-718-7
NACRES:
NA.32
Form:
lyophilized powder
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Quality Level

200

product line

BioReagent

form

lyophilized powder

potency

<40 μg per mL agglutination activity

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

solubility

PBS: 2 mg/mL

storage temp.

2-8°C

Application

Lectin from Phaseolus vulgaris (red kidney bean) is used in Lymphocyte Transformation Assay and culture medium.[1]

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.

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This Item
L1668L4144L2769
Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

L8902

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

Sigma-Aldrich

L1668

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

L4144

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Leucoagglutinin PHA-L, lyophilized powder

Sigma-Aldrich

L2769

Lectin from Phaseolus vulgaris (red kidney bean)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

form

lyophilized powder

form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

form

lyophilized powder

form

lyophilized powder

impurities

salt, free

impurities

-

impurities

salt, free

impurities

salt, free

solubility

PBS: 2 mg/mL

solubility

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

solubility

DPBS: 5 mg/mL, slightly hazy, colorless

solubility

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

potency

<40 μg per mL agglutination activity

potency

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.), ≤1.0 μg per mL leucoagglutination activity, ≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.), ≤1.0 μg/mL leucoagglutination activity, ≤5 μg/mL mitogenic activity

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000524493
0000524493
0000524494
0000524494
0000524495

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Related Content

Data Sheet - L8902

Instructions

J Kabeerdoss et al.
Alimentary pharmacology & therapeutics, 34(8), 923-930 (2011-08-19)
Helminths have been used to inhibit intestinal inflammation in patients with Crohn's disease. This study was undertaken to determine if there is a protective association of prior hookworm infection with Crohn's disease, in a region where there is epidemiological transition
Pinar Ozkal et al.
Mutagenesis, 20(2), 147-150 (2005-04-22)
Chronic viral hepatitis is the main cause of chronic liver disease, cirrhosis and hepatocellular carcinoma throughout the world. Hepatitis B virus (HBV) has mutagenic effects on somatic cells. HBV may be showing these mutagenic effects through its viral proteins or
Stefan Warmuth et al.
Oncoimmunology, 10(1), 2004661-2004661 (2022-07-19)
Co-stimulatory 4-1BB receptors on tumor-infiltrating T cells are a compelling target for overcoming resistance to immune checkpoint inhibitors, but initial clinical studies of 4-1BB agonist mAbs were accompanied by liver toxicity. We sought to engineer a tri-specific antibody-based molecule that
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Global Trade Item Number

SKUGTIN
L8902-100MG04061833966228
L8902-5MG04061835557639
L8902-25MG04061833966242

Questions

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

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