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H8264

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

Hanks’ Balanced Salt solution

Synonym(s):

HBSS

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
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Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: no
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

shipped in

ambient

storage temp.

room temp

General description

Hanks’ Balanced Salt solution (HBSS) is a buffer that mimics biological medium and stabilizes cells. It is useful in leucocyte labeling studies. HBSS is an effective preservative for periodontal ligament cells.[1] HBSS lacks essential inorganic ions and is useful in starvation studies.[2]

Application

Calcium and magnesium are included in this formulation and support cell adhesion. Used to minimize cell attachment or aggregation.


Hanks’ Balanced Salt solution has been used:

  • as a rinse solution for hepatocytes[3]
  • for hippocampi dissection[4]
  • in corrosion testing of dog-bone specimens[5]
  • for starvation studies in breast cancer cell line[2]
  • as a component of dissociation solution for dorsal root ganglion[6]
  • as a chamber solution for the patch-clamp apparatus

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

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This Item
H6648H9394H9269
Hanks’ Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

H8264

Hanks′ Balanced Salt solution

Hanks’ Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

H6648

Hanks′ Balanced Salt solution

Hanks’ Balanced Salt solution Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

H9394

Hanks′ Balanced Salt solution

Hanks’ Balanced Salt solution With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

H9269

Hanks′ Balanced Salt solution

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

components

phenol red: no
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

components

NaHCO3: 0.35 g/L
phenol red: 0.011 g/L
glucose: 1.0 g/L (Dextro)

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0244
RNBL7910
RNBL6002
SLCN1542
RNBL5109

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Physical removal of the midbody remnant from polarised epithelial cells using take-up by suction pressure (TUSP)
Bernabe-Rubio,M, et al
Bio-protocol, 7 (2017)
Emmet Galvin et al.
Medical & biological engineering & computing, 55(8), 1261-1275 (2016-10-28)
Magnesium stents are a promising candidate in the emerging field of absorbable metallic stents (AMSs). In this study, the mechanical and corrosion performance of dog-bone specimens and a specific stent design of a magnesium alloy, WE43, are assessed experimentally in
Hank s balanced salt solution: an alternative resuspension medium to label autologous leukocytes. Experience in inflammatory bowel disease
Martin-Comin J, et al.
Brazilian Archives of Biology and Technology, 45, 39-44 (2002)
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Global Trade Item Number

SKUGTIN
H8264-200B04061842649662
H8264-100ML04061833807620
H8264-1L04061833808047
H8264-6X500ML04061833808610
H8264-1LSTAB04065272732116
H8264-500ML04061833808177
H8264-6X1L04061833808467
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Questions

  1. Can this be used for testing bioglass in terms of hydroxyapatite growth?

    1 answer

    1. This product has not been tested for the use in bioglass in terms of hydroxyapatite growth. Application specific use of this product needs to be determined by the end user. The formulations of this and other HBSS products are provided below for further evaluation: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/555/001/h8264for.pdf

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