H7166

Interleukin-3 human

IL-3, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture

• Synonym(s): IL-3
• MDL number: MFCD00147276
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in HEK 293 cells
• Assay: >95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤2 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 17-45 kDa (glycosylated)
• packaging: pkg of 10 μg
• manufacturer/tradename: HumanZyme
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/μg
• UniProt accession no.: P08700
• storage temp.: −20°C
• Gene Information: human ... IL3(3562)

Description

Biochem/physiol Actions

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine Interleukin-3 (IL-3), expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 17-45 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation which contributes to stability in cell growth media and other applications.

Analysis Note

The activity was determined by the dosedependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable