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E2412

Pullulanase microbial

Synonym(s):

Pullulanase microbial, Promozyme® D2

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About This Item

CAS Number:
9075-68-7
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.2.1.41 (BRENDA, IUBMB)
MDL number:
MFCD00081941
Specific activity:
≥1000 NPUN/g
Recombinant:
expressed in Bacillus subtilis
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recombinant

expressed in Bacillus subtilis

Quality Segment

200

form

aqueous solution

specific activity

≥1000 NPUN/g

storage temp.

2-8°C

Application

Pullulanase is a glucanase that degrades pullulan. It is commonly used for starch-debranching as well as to study polysaccharide utilization[1].

Biochem/physiol Actions

Type I pullulanases specifically hydrolyse α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Legal Information

A product of Novozymes Corp.
Novozym is a registered trademark of Novozymes A/S
Promozyme is a trademark of Novozymes Corp.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
0000560760
0000528323
0000528323
0000560760
0000489804

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Global Trade Item Number

SKUGTIN
E2412-50ML04061833658321
E2412-250ML04061833602614

Questions

1–7 of 7 Questions  

  1. What is the optimum pH and media solution for Pullulanase activity?

    1 answer

    1. The Enzymatic Assay for Pullulanase is described in full detail here:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/517/754/pullulanase.pdf?srsltid=AfmBOoowPYNfQjzj1eyi5czeqN9kE7fYBqx-72QH5gfC3fOBMAbjm36v

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  2. What is the meaning of NPUN/g of pullulanase enzyme E2412?

    1 answer

    1. The activity is expressed as NPUN/g, which refers to New Pullulanase Unit Novozymes (NPUN) per milligram of protein. The international unit correspondence is described as follows: 1 NPUN = 0.35 PUN (Pullulanase Unit Novozymes), where 1 PUN is defined as the amount of enzyme that, under standard conditions, releases 1 ?mol of glucose per minute.

      Helpful?

  3. I was wondering the proportion of type 1 and type2 in pullulanase?

    1 answer

    1. Product Code E2412 contains both Pullulanase 1 and 2, but the exact ratio is considered proprietary.

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  4. May I know how to make 10units/mL sample from the bottle?

    1 answer

    1. The activity in the batch-specific Certificate of Analysis is expressed as NPUN/g. The international unit correspondence is described as follows: 1 NPUN = 0.35 PUN, where 1 PUN is defined as the amount of enzyme that releases 1 μmol of glucose per minute under standard conditions.

      The Certificate of Analysis also reports the density (g/mL) of the product. To calculate the activity in units per milliliter (UPUN/mL), multiply the activity (NPUN/g) by the density (g/mL). Further dilution can then be performed to achieve the desired final concentration.

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  5. Could you let me know if there are other substances included in this product for stabilization purposes, aside from the pullulanase enzyme? If so, could you specify what those additives are?

    1 answer

    1. The composition of this product is considered proprietary by the supplier, and the components have not been provided. For inquiries about the composition, please contact the supplier directly.

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  6. I was wondering if product E2412-50ML is a type 1 or type 2 pullulanase (degrades alpha1,6 linkages only or both alpha1,4 and 1,6?)

    1 answer

    1. E2412 contains both Type 1 and Type 2 Pullulanases.

      Helpful?

  7. What is the molecular weight of this Pullulanase enzyme? And is there any glucose in the enzyme solution?

    1 answer

    1. The molecular weight has not been determined, however, a search of the literature indicates that the general molecular weight of Pullulanase, CAS 9075-68-7, ranges from 70 - 115 kDa. This is a product of Novozymes Corp. The manufacturer considers the formulation of this product proprietary but has noted that this material is pure Oligo-1,6-Glucosidase.

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