C0743

Anti-Calsequestrin-1 (N-terminal) antibody produced in rabbit

affinity isolated antibody

• Synonym(s): Anti-Aspartactin, Anti-CALMITINE, Anti-CASQ1, Anti-Calsequestrin-skeletal muscle isoform
• UNSPSC Code: 12352203
• NACRES: NA.44
• MDL number: MFCD09265356
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 11

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: rat, mouse, bovine (predicted), human (predicted)
• concentration: ~1.0 mg/mL
• technique(s): western blot: 0.5-1 μg/mL using extract of rat skeletal muscle S1 fraction
• UniProt accession no.: P31415
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CASQ1(844) ; mouse ... Casq1(12372)

Description

General description

Calsequestrin (CS also known as CSQ), the major Ca²⁺ binding protein in cardiac and skeletal muscle, is a high-capacity, low-affinity Ca²⁺ binding glycoprotein, which functions as an internal Ca²⁺ store in the lumen of the SR. In mammalians, two forms of the protein are found, calsequestrin-1 (CASQ-1) and calsequestrin-2 (CASQ-2), which encode the fast-twitch skeletal muscle and cardiac calsequestrin, respectively. Calsequestrin-1 is found in the SRs terminal cisternae luminal space of fast skeletal muscle cells. Calsequestrin-1 is a putative autoantigen associated with eye muscle inflammation in Graves′ disease.

Immunogen

synthetic peptide corresponding to amino acids 28-45 located near the N-terminus of mouse calsequestrin-1, conjugated to KLH. This sequence is identical in rat and highly conserved in human and bovine calsequestrin-1 (two amino acid substitution). This sequence is not found in calsequestrin-2.

Application

Anti-Calsequestrin-1 (N-terminal) antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using extracts of rat skeletal muscle S1 fraction.

Biochem/physiol Actions

Calsequestrin (CS) is a calcium-binding protein in the sarcoplasmic reticulum (SR). It is known as CSQ and PDIB1. The gene belongs to calsequestrin protein family. Calsequestrin-1 is a putative auto-antigen associated with eye muscle inflammation in Graves′ disease. Calsequestrin-2 (55 kDa) is found in the SR′s terminal cisternae luminal space of cardiac, slow skeletal muscle cells and may be in platelets. The major Ca2+ binding protein in cardiac and skeletal muscle is a high-capacity, low-affinity Ca2+ binding glycoprotein, which functions as an internal Ca2+ store in the lumen of the SR. It helps in controlling the smooth-muscle contraction.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)